of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the reaction on the pectinase
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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20 9. Classic chemistry experiments Unsaturation in fats and oils Topic Organic chemistry‚ saturated and unsaturated fats. Timing 45 min. Description The students titrate different oils and fats mixed with Volasil against bromine water. Apparatus and equipment (per group) w White tile w Conical flask w Dropper pipette. (Use the type of teat pipette usually fitted to Universal Indicator bottles‚ that do not allow squirting – eg Griffin.) w Burette (filled with bromine
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Experiment three was divided into three parts; the preparation of the solutions‚ the reaction between Hydrochloric Acid and Ammonium Hydroxide (part b)‚ and the formation of Magnesium Oxide (part c). 200 mL of deionized water were added to a beaker followed by the addition of 100 mL 6 M HCl‚ which reacted to make 300 mL of a 2 M HCl solution used for Part B. Next‚ 50 mL of deionized water were added to a separate beaker and then 100 mL 3 M NaOH were added to the beaker to form 150 mL of a 2 M NaOH
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The SN2 reaction requires three qualities: a strong nucleophile‚ a good‚ unhindered leaving group‚ and a polar‚ aprotic solvent. For our reaction‚ we have all three bases covered. The nucleophile is an alkoxide‚ a deprotonated alcohol. Technically‚ because our alcohol is a phenol‚ the conjugate base is called a phenoxide. Phenol itself has a pKa of about 10‚ but our alcohol has more resonance opportunities‚ so the pKa is down around 8. This is sufficiently acidic for use of weak base like K2CO3 for
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Diels-Alder reaction was performed in this experiment. Which is a reaction that was discovered by Otto Diels and Kurt Alder. This particular type of reaction is the concerted cycloaddition among a dienophile and a diene. The Diels-Alder reaction allows for the synthesis of stereospecific rings in an efficient manner. This reaction proceed in a single step method‚ otherwise known as Nucleophilic Substitution in the second order (SN2). The Diels-Alder reaction is categorized as a pericyclic reaction‚ this
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Link reaction The link reaction takes place in the mitochondrial matrix and its main function is to turn the pyruvate into acetate for the kerb cycle. During the link reaction the pyruvate molecule undergoes decarboxylation and dehydrogenation‚ the enzymes pyruvate decarboxylase and pyruvate dehydrogenase remove the carboxyl group (which becomes a carbon dioxide molecule) and removes the hydrogen atoms from the pyruvate molecule. The coenzyme NAD accepts the two hydrogen atoms and becomes reduced
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Determining the Concentration of an Unknown Solution Graph: The effect of concentration of CuS04 * 5H20 on transmittance. I solved for my unknown by plugging in “y” as my given transmittance value‚ which was 85.0. y = -278x + 100.12 .85 = -278x + 100.12 -99.27 = -278x X = .357M (concentration) The relationship between the transmittance values and the concentration is an indirect relationship. Whenever the concentration increases‚ the transmittance decreases. It can also be reversed
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Title of the Experiment: determination of densities Introduction The density of a sample of matter represents the mass contained within a unit volume of space in the sample. For most samples‚ a unit volume means 1.0 ml. The units of density‚ therefore‚ are quoted in terms of grams per milliliter (g/ml) or grams per cubic centimeter (g/cm3) for most solid and liquid samples of matter. Density is often used as a point of identification in the determination of an unknown substance. The density
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Name: Taylor Wright Lab Partners: Cody‚ Jeremy‚ Haly‚ Eric‚ Brody Date: October 14th Class: Chemistry 20 Charles’ Law Lab Introduction: The purpose of this investigation is to demonstrate how water temperature affects the volume of a balloon. According to Charles’ Law‚ temperature and volume increase proportionally‚ as long as chemical amount and pressure remain the same. Research Question: How does the volume of a balloon react to changing temperatures? Hypothesis: If we increase the temperature
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