BIOLOGY HSC NOTES MAINTAINING A BALANCE • Identify the role of enzymes in metabolism‚ describe their chemical composition and use a simple model to describe their specificity on substrates. Enzymes are protein molecules that allow the body to engage in chemical reactions‚ such as metabolism. There activities can be catalytic (being able to control the rate of either increasing/decreasing chemical reaction) Enzymes have a specific shape‚ and this shape must be intact‚ otherwise the effectiveness
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Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the
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certain environmental factors affect the enzyme activity rate. For the first experiment‚ where we tested the increase in concentration of enzyme with the substrate‚ we found that higher concentration of enzyme increases the rate of reaction of the enzyme. This is because more enzyme molecules are present‚ which allow more substrate molecules to get into the active sites of the enzyme (Sattler W& Esterbauer H). When calculating the absorbance of different enzyme concentration‚ it was noticeable that
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Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and some
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Biology Enzymes IA Design Introduction: Enzymes are globular proteins‚ they are responsible for most of the chemical activities of a living organism. They act as catalysts‚ substances that affects the reaction of other substances without being destroyed or altered during the process. They are extremely efficient in the body system of living organisms‚ one enzyme may catalyse over a thousand chemical reactions every second. But there are certain conditions that need to be fulfilled in order for the
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TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost all of the chemical reactions in living organisms. Without enzymes‚ these
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Omar Shbeeb Toothpick Enzyme Lab 9/25/13 Introduction Enzymes are used in all metabolic reactions to control the rate of reactions and decrease the amount of energy necessary for the reaction to take place. They are responsible for the thousands of chemical interconversions that sustain life. Enzymes are referred to highly selective catalysts‚ meaning they speed up the rate of metabolic reactions. To react‚ they need to find a perfect match with a substrate. They converge at a place called an
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Broadly speaking‚ enzymes are proteins that is produced to perform as a biological catalyst in chemical reactions. Catalyst are used to increase the rate of a chemical reaction. In this study‚ we performed two different experiments that investigated the effect of varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis
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The Tragedy of Commons Bite-sized trash were found in the North Pacific Ocean‚ known as the Great Pacific Garbage Patch. Scientists from the University of California found more plastic bits than anticipated‚ and a San Diego press conference was held to document the harmful impacts of the trash on coastal marine life. Some trash travels a long journey all the way to the center of the ocean. They float on the surface and release potentially toxic chemicals while decomposing‚ bring disruption to marine
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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