Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography
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Purpose: In this lab‚ a mixture of naphthalene (C10H8)‚ common table salt (NaCl)‚ and sea sand (SiO2) will be separated using the separation techniques in order to demonstrate the properties of mixtures and their ability to be separated by physical means. INTRODUCTION: This lab was based on the separation of the components of a mixture. A mixture can be defined as a physical combination of two or more pure substances. Separation techniques are used to separate components that are not chemically
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Post Lab #4- Column Chromatography Organic Chem 3418-2 March 3‚ 2011 Theoretical Background- The fluorene and fluorenone mixture was separated by first dissolving the mixture in heptane. Since “like dissolves like”‚ fluorene dissolves with the non-polar heptane and the polar fluorenone dissolves in the polar ethyl acetate solvent. This phenomenon was illustrated in class before the experiment‚ when it was pointed out why water will not dissolve fluorene‚ fluorenone‚ or transstilbene
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GAS LAWS Properties of Gases Gases expand into any available volume • gas molecules escape from open containers. Gases are completely miscible • once mixed they will not spontaneously separate. Gases are described in terms of T‚ P‚ V and n Volume‚ Amount and Temperature • A gas expands uniformly to fill the container in which it is placed – The volume of the container is the volume of the gas – Volume may be in liters‚ mL‚ or cm3 • The temperature of a gas must be indicated
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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separation of the components of a mixture. A mixture can be defined as a physical combination of two or more pure substances. Separation techniques are used to separate components that are not chemically combined. All of these techniques involve changes in the physical state of a chemical compound rather than chemical changes. Sea sand‚ table salt‚ and two other unknown substances will be separated using the separation techniques in order to demonstrate the properties of mixtures and their ability to be separated
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Laboratory 4: Separation of a Mixture of Solids Numbers 1 to 4 below are not written out in your Lab Manual. They are provided here to help you do your calculations more clearly. 1. Separating out the Iron: Mass of Weighing Dish: ___0.6_______g (Read all masses to the decimal places allowed by the balance/scale‚ typically one or two decimals‚ i.e. a tenth or a hundredth of a gram.) Mass of Weighing Dish plus Solids Mixture: ____7.5______ g Mass of Solids Mixture: ___6.9_______ g Mass
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Experiment 1: Mixtures Aim: To develop an understanding of different types of mixtures including solutions and to examine the different solubilities of some solutes in two solvents: water and ethanol Procedure: Refer to Experiments Book Pg 13 Results: Part A- Mixture Observations Classifications 1 100% Orange Juice There were suspended pulp in the juice Heterogeneous Mixture 2 100% Apple Juice Clear solution Homogeneous Mixture 3 Solder Uniform mixture Homogeneous Mixture 4 Fruit Cake Chunks
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Separation of a Mixture of Solids Lab: Separation of a Mixture of Solids Experiment PURPOSE The purpose of this experiment was to learn how to separate a mixture of solids. DATA | | |Data Table 1: Experiment Data | | |Grams |Percent of mixture | |Iron filings |2.2
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