Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex mixtures. Using
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ALCOHOLS‚ ETHERS‚ AND PHENOLS Juris Marie G. Garcia Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Date Performed: February 27‚ 2015 Date Submitted: March 06‚ 2015 Answers to Questions: 1.) Balanced equations: A. Lucas Test Test Sample Equation Ethyl Alcohol C2H5OH + HCl + ZnCl2 C2H5Cl + H2O Isopropyl Alcohol (CH3) 2CHOH + HCl (CH3) 2CHCl + ZnC2 + H2O Tert-butyl Alcohol (CH3) 3COH + HCl + ZnCl2 (CH3) 3CCl + ZnCl2 +H2O Benzyl Alcohol C6H5CH2OH + HCl + ZnCl2
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exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography‚ in
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Gas analysis techniques Terms for gas analysis techniques Concentration The term concentration describes the amount of a substance‚ expressed as mass‚ volume‚ or number of particles in a unit volume of a solid‚ liquid‚ or gaseous substance e.g. alcohol in beer or oxygen in air. Different units are in use to describe concentration in gases: Mass concentration Concentration expressed in terms of mass of substance per unit volume [g substance/m3 gas volume] ¢ Volume concentration Concentration
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chemistry‚ an alcohol is an organic compound in which the hydroxyl functional group (-OH) is bound to a carbon atom. In particular‚ this carbon center should be saturated‚ having single bonds to three other atoms.[1] An important class of alcohols are the simple acyclic alcohols‚ the general formula for which is CnH2n+1OH. Of those‚ ethanol (C2H5OH) is the type of alcohol found in alcoholic beverages‚ and in common speech the word alcohol refers specifically to ethanol. Other alcohols are usually
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separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy to synthesize carbohydrates from CO2 and water
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The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation
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How much alcohol do teens use? Alcohol is the most frequently used drug by teenagers in the United States. About half of junior high and senior high school students drink alcohol on a monthly basis‚ and 14% of teens have been intoxicated at least once in the past year. Nearly 8% of teens who drink say they drink at least five or more alcoholic drinks in a row (binge drink). What are the dangerous effects of alcohol use in teens? Just a few of the many dangerous effects of alcohol use in teens
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Alcohol Facts and statistics ( www.niaaa.nih.gov-2012 ) Global Burden of alcohol abuse‚ In 2012‚ 3.3 million deaths‚ or 5.9 percent of all global deaths (7.6 percent for men and 4 percent for women)‚ were attributable to alcohol consumption.. Alcohol contributes to over 200 diseases and injury-related health conditions‚ most notably alcohol dependence‚ liver cirrhosis‚ cancers‚ and injuries. In 2012‚ alcohol accounted for 5.1 percent of disability adjusted life years (DALYs) worldwide. Globally‚
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Introduction Atoms‚ molecules‚ compounds and mixtures‚ although they may not seem it‚ are different. Atoms make up all of the above and elements. They are made an equal amount of protons and electrons. Molecules are made when two or more atoms connect chemically. Like molecules‚ compounds are made when two or more atoms connect chemically. Unlike molecules‚ the atoms need to be two or more different elements. Mixtures are made of two or more compounds or elements. Mixtures can look completely the same when
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