Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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Introduction: The purpose of this lab is to see how the colour of an apple would change (browning) over time‚ when placed in different conditions. When referring to the term “browning”‚ it is meant to imply the change of colour that occurs inside the apple‚ giving an appearance that is distasteful (Di Guardo et al.‚ 2013). Specifically‚ within this lab the apple was cut into 4 pieces with 3 pieces being placed on a weigh boat‚ and a 4th piece placed in a beaker of water. These weigh boats were
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I also hypnotized that the enzyme is acidic on the pH scale because it exists in our small intestine and our small intestine uses acid to break down food. The result from the lab prove my hypothesis correct because lactase worked well at body temperature and it also worked well at pH of 4.5 to 5.5 mg/dl proving that lactase works best at an acidic pH number. The lab shows that the highest amount of glucose is made by temperatures close to body temperature. In our our lab‚ we found that at 35 °C 500
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Investigating the effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which is
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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[Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on the desired
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BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
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it’s lowest to do so. However‚ in cold solutions the starch will take longer as it will in temperatures beyond 40 degrees. Once it reaches this point‚ the break down will either take a very long period of time or have no reaction at all as enzymes are denatured at a certain point. Materials: · 4 x test tubes · 5mL Diastase · 5mL Water · 10mL 2% Starch Suspension. · Pipette · 2 x Spotting tiles · Large Beaker filled with water of assigned temperature · Thermometer
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Enzymes (pron.: /ˈɛnzaɪmz/) are large biological molecules responsible for the thousands of chemical interconversions that sustain life.[1][2] They are highly selective catalysts‚ greatly accelerating both the rate and specificity of metabolic reactions‚ from the digestion of food to the synthesis of DNA. Most enzymes are proteins‚ although some catalytic RNA molecules have been identified. Enzymes adopt a specific three-dimensional structure‚ and may employ organic (e.g. biotin) and inorganic (e
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