can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes; The enzyme catalase has an optimum operation
Premium Enzyme Metabolism Catalysis
Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle‚ particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions‚
Premium Enzyme
An enzyme is a tertiary globular protein. The function of an enzyme is to lower the activation energy of either the creation or breaking apart of a chemical bond. By lowering the activation energy of this process‚ the reaction of bonding‚ or in this case breaking apart‚ is sped up. An enzyme breaks apart the substrate in the active site of the enzyme; this is where the magic happens. Substrate is what is being broken apart by the enzyme. In this case‚ the enzyme is catalase and the substrate is hydrogen
Premium Enzyme Catalysis Chemical reaction
Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
Premium Diffraction Wavelength Light
LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
Premium Plasmid Transformation Escherichia coli
PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
Premium Oxygen Carbon dioxide Ratio
CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
Premium Enzyme Enzyme inhibitor
Neurophysiology Lab Report Anatomy & Physiology Lab Report Exercise 3 Activities 1-4‚ 8 By Laurence Blake 2/27/12 A. Objective I. Activity 1-4: Eliciting a Nerve Impulse • Investigate what kinds of stimuli stimulate action potential. II. Activity 8: Nerve Conduction Velocity • Determine and compare the conduction velocities of different types of nerves. B. Introduction I. Activity 1-4: Eliciting a Nerve Impulse • In this experiment‚ we
Premium Hydrochloric acid Nerve Action potential
concentration of dilute solution V2 = volume of dilute solution Chemicals 0.100 M FeCl3 solution 0.100 M KCNS solution 0.005 M FeCl3 solution (B) Apparatus Burette 1 x 50 mL Pipette 1 x 5 mL Test tube 10 2.3: ACTIVITIES Method: 1. Based on given equation‚ calculate and prepare the following solution from standard solution of 0.100 M FeCl3 by using pipette. a. 25mL FeCl3 solution (5.0 x 10-2 M) b. 25mL FeCl3 solution (1.0 x 10-2 M) c. 25mL FeCl3 solution
Premium Concentration Chemistry
Contact: Incep Bio values your feedback and queries related to our company‚ services‚ capabilities‚ and experience. We are fully committed to support the industries that we serve to come across the complete and most cost effective solutions to meet their requirements. Let us know how Incep Bio can help and one of our specialists will quickly reply to your queries. FAQ: 1. Who is INCEP BIO? Incep Bio Consulting Laboratories‚ Inc. is a GLP/GMP compliant laboratory founded in 1980. Our professional
Premium Management Strategic management Customer service