Cellular Biology Lab – Homework #3 Due to the week of Nov. 10-14th You may use the lab manual‚ pre-lab lectures‚ and credible internet resources‚ however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA.Plagiarism will result in an automatic zero. 1. [15pt]In the cell bio lab‚ we use company manufactured gels‚ however you can make you own polyacrylamide
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H Week 8 Week 8 Lab: Molecular Biology Review Test Submission: Molecular Biology Lab Assessment Review Test Submission: Molecular Biology Lab Assessment Us er Cours e Introduction To Biology Tes t Molecular Biology Lab As s es s m ent Started 8/19/14 10:14 AM Subm itted 8/19/14 10:22 AM Status Com pleted Attem pt Score 27 out of 30 points Tim e Elaps ed 7 m inutes out of 2 hours . Ins tructions
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amino acid sequence in humans to the sequences of the other organisms listed. For each organism‚ identify any amino acid that is different or missing when compared to the amino acids in the human sequence. 1. Click on the following link to open the activity chart: Amino Acid Sequences in Cytochome-C Proteins. 2. Compare the human/chimpanzee to each other organism‚ entering the number of differences in the chart (Tip: the next comparison is human/chimpanzee vs. horse). Note that a minus sign (-) indicates
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ESTIMATION OF 1-4‚ DIOXANE IN SHAMPOOS/ CONDITIONERS/HAIR CREAMS/ SODIUM LAURETH SULFATE BY HEAD SPACE GAS CHROMATOGRAPHIC TECHNIQUE INTRODUCTION 1-4‚ DIOXANE 1‚4-Dioxane is a heterocyclic organic compound‚ classified as an ether. It is a colorless liquid with a faint sweet odor similar to that of diethyl ether. The compound is often called simply Dioxane because the other Dioxane isomers (1‚2- and 1‚3-) are rarely encountered. CHEMISTRY OF 1-4‚ DIOXANE
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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yellowish-green color. When there is a presence of something that is acidic the BTB indicator changes its color into yellow/green. So in this case‚ the water with BTB and carbon dioxide exhaled into the flask creates a carbonic acid. As an aerobic activity increase than the carbon dioxide rate increases as well. 2. By exercising more‚ one’s body requires more energy in their muscles. As the exercise becomes more continual‚ the aerobic respiration is primarily needed. Due to aerobic respiration there
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synthesis of 4-hydroxy-4‚4-diphenylbutn-2-one was a three week multi-step project. The steps involved protecting the ketone of ethyl acetoacetate with a ketal group‚ followed by preparation and reaction with Grignard‚ then purification by extraction and recrystallization. The yield was 78.5%‚ but there are impurities present suggested by 1H-NMR‚ IR‚ and TLC. However‚ the results do indicate the desired hydroxyketone was formed. Introduction: The purpose of this multi-step lab was to synthesize 4-hydroxy-4
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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat is a
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Misael Acosta Photosynthesis Lab Report Ms. Rocha AP Biology 4th Period II. Intro/Background 1. In order for oxygen to be present photosynthesis must occur. Photosynthesis takes in light and CO2‚ and through a series of steps produces oxygen. Photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. Examples include the production of O2 or the consumption of CO2. All the oxygen within the leaf disks will be removed before being placed
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