Identifying Organic Compounds 2. Purpose/Problem: I am doing this experiment to find out what kind of organic compounds are in test tubes A‚ B‚ C and D. I am curious to know if substance D was the lipid because it seemed thin and had a slightly yellow tint of color to it. 3. Research: In my research I was informed about facts and appearances of lipids. First of all lipids are non-polar compounds‚ thus they won’t dissolve in water. This is also known as being hydrophobic. They are made with one
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presence of monosaccharides by using the Benedict’s test Test for the presence of starch‚ a polysaccharide‚ by using the Lugol’s iodine test Test for the presence of lipids by using the Sudan test Test for the presence of proteins by using the Biuret reagent test Determine the identity of an unknown substance by detecting the presence of organic macromolecules using the above tests Introduction Biological macromolecules are defined as large molecules made up of smaller organic molecules. There
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indicating the presence of starch. The Iodine solution traps itself in the helical structure of the polysaccharide to form dark blue colour. 3. To test for the presence of glucose‚ Benedict’s solution was used to test for reducing sugars. Benedict’s reagent is clear blue due to the copper
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protein‚ 1% glucose‚ and distilled water (DW)‚ slurries of honey‚ egg white‚ and saltine crackers dropper of IKI solution (starch test) dropper bottle of Benedict’s reagent (glucose test) dropper bottles of 10% NaOH and 2% CuSO4 (Biuret Reagents – protein test) 95ºC water bath masking tape for labeling test tubes Procedure: Biuret test for a typical protein: Place a small piece of masking tape on 7 clean test tubes. Using a permanent marking pen‚ label the tubes 1-7. Add drops of solutions
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-Baking soda solution‚5% -Glucose solution‚ 5% -Known foods; glucose‚ corn starch -Cornstarch suspension‚5% -Detergent solution -Unknown food samples -Benedict’s reagent -Lugol’s solution -Sudan IV indicator -Unglazed brown paper -Gelatine solution‚ 5% -Biuret reagent Procedure: 1. Safety goggles and lab apron were put on. Part A: Carbohydrate Test 2. Poured 300 mL of water into a 400 mL beaker. Secured the beaker with a ring clamp‚
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through fused rings‚ their function is to carry cholesterol‚ phytosterol‚ and hormones. The Biuret Reagent test is a straightforward test to identify lipids. To perform this test‚ one must combine equal amounts of a lipids and Biuret Reagent solution‚ let it set for several minutes before analyzing. Once the mixture has settled into its color‚ take a look‚ the substance should have the blue Biuret Reagent solution settled at the bottom and the lipid floating clearly on
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up‚ with one set for each of the following sample solutions: water‚ protein‚ starch‚ lipid‚ vitamin C and an unknown sample. 0.5 L of each solution was added to each tube. One drop of each of the three indicator solutions (iodine‚ Sudan III and Biuret & copper sulfate) was added to each sample solution to determine how that sample solution (and that type of macromolecule) would react with each specific indicator. The results were recorded in the data chart below. The identity of the unknown solution
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Q 1. In this practical exercise you will be using biuret reagent. Why is biuret reagent used in this practical? The biuret reagent is used to assess the concentration of the protein because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the colour‚ and therefore the absorption‚ is directly proportional to the protein concentration. Q 2. What is the experimental reason for constructing a standard curve? Multiple samples with known properties
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BIOL 140 Lab—The Chemical Composition of Cells Name ____________________________ I) Introduction All cells contain four major types of macromolecules: carbohydrates‚ lipids‚ nucleic acids‚ and proteins. In today’s lab‚ we will be studying three of the four-proteins‚ carbohydrates and lipids. Various chemical tests can be used to detect the presence of each of these molecules. Most of the tests involve a color change visible to the eye. If a color change is observed‚ the test is considered positive
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team searched for the presence of starch‚ glucose‚ lipids‚ and proteins through the use of indicators: Sudan III‚ Lugol’s iodine‚ Benedict’s solution‚ and Biuret’s reagent. After testing the gelatin solution in the presence of the indicators‚ the results showed that Lugol’s reacted‚ turning orange-pink (oils) and the Biuret reagent (protein) reacted with the gelatin solution‚ turning lilac. Thus it was determined that the gelatin solution held both proteins as well as oils. Introduction: Lipids
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