The effect of Lead ions on amylase activity Aim What is the effect of Lead ions on the enzyme Amylase. And does it have an inhibitory effect‚ which causes the substrate‚ in this case starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible‚ which is put on the amylase. Method Apparatus and substances required Test tube holder 2% starch solution 6 boiling tubes labelled 1 to 6 1% lead nitrate solution 6 test tubes labelled A to E‚
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glucose from red blood cells pass the cell membrane and into the cell. Enzymes are inhibited by a variety of organisms. These organisms are called competitive inhibitors or noncompetitive inhibitors. Competitive inhibitors inhibit enzymes on their active site so that the substrate cannot fit into the active site. Noncompetitive inhibitors bind to a site other than the active site thus changing the shape of the enzyme to the point that it looses affinity for the substrate. (Giuseppe‚ M 2002 p. 73)
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Barrett Browning’s How Do I Love Thee? Introduction Elizabeth Barrett Browning’s How Do I love Thee‚ or Sonnet XLIII is one of her love poems from Sonnet from the Portuguese (1850). This is the manuscript she slipped into her husband’s (Robert Browning) pocket one morning after breakfast‚ and was originally intended as a private gift. When she finished Sonnets from the Portuguese in 1847‚ the book had no title. At that time‚ the couple was staying in Italy. Mostly the main idea in this series of
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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comfort long‚ and lose thy love thereby! But love me for love’s sake‚ that evermore Thou mayst love on‚ through love’s eternity. ------------------------------------------------- Analysis In lines I and 2 of "Sonnet 14"‚ Elizabeth Barrett Browning says she wants only to be loved for "love’s sake". The next four lines describe all the things she does not want to be loved for. She tells us in lines 7 through 9‚ that she does not want to be loved for these reasons because they are changeable
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enzyme-substrate complex. Inhibition can also affect enzymes‚ there are two types of inhibitors they are the competitive and non-competitive. A competitive inhibitor has a shape similar to the substrate. This means that they compete for the active site and block it off so that no substrate can fit in to it. A non-competitive inhibitor has its own site which changes the shape of the active site ones the inhibitor changes the active site no substrate can fit in to make an end product. Some plants
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Part 1A Analysis questions: 1. How many “chainobeads” was your enzyme able to make per minute in the 0 – 15 second interval? Our enzyme was able to make 6 chainobeads in the 0-15 interval. 2. How many “chainobeads” was your enzyme able to make per minute in the 60 – 120 second interval? Our enzyme was able to make 49 chainobeads in the 60-120 intervals. 3. Did your enzyme’s rate change over time? How does this compare to a real enzyme? The enzyme’s rate did change over time. This compares to a
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Chapter 8 Metabolism Thousands of biochemical reactions All require Metabolism transforms matter & energy & obeys the laws of physics Several steps from starting reactant to product Metabolic Pathway 3 steps Each is a separate reaction carried out by a unique and specific enzyme Catabolic Break down complex molecules into simpler ones Release energy Anabolic Build molecules up Consume energy Energy The capacity to cause change Many forms in which some we can use to do work Convert energy from one
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SONNET 13 In the first two lines of "Sonnet 13"‚ Elizabeth Barrett Browning asks Robert if he wants her to write how she feels about him. In lines 3 and 4‚ she uses the metaphor of a torch in rough winds‚ which is meant to enlighten what is between them. In line 5‚ she drops it and goes on to say she cannot describe what she feels between them. In lines 6 through 8‚ she says she cannot risk herself by describing to him how she feels‚ and that she will not. In lines 9 through 14‚ she goes on to say
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such offense as their foundation for their production of literature. I will base the bulk of my argument around the author Charlotte Bronte and her novel “Villette” while simultaneously using additional sources such as that of Elizabeth Barrett Browning to further verify my position. In 1837‚ R. Southey‚ in a response to Charlotte Bronte’s poetry‚ stated that; “Literature cannot be the business of a woman’s life‚ and it ought not to be. The more she is engaged in her proper duties‚ the less
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