cells of all living organisms such as hormones‚ enzymes‚ antibodies. [1] Fig. 1 Fig. 2 General structure of amino acids Protein fragment showing peptide bond Basis of Spectrophotometer Measuring amount of substance in solution by helping of solution color is called colorimetry. Devices that used for this method is called colorimeter. In colorimetric measurements‚ color of solution is compared with color of standard solutions
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Objectives: 1. To determine the wavelength of maximum absorption‚ Amax‚ of bromophenol blue. 2. To construct a standard concentration curve of bromophenol blue. 3. To determine the concentration of the unknown bromophenol blue solutions. 4. To determine the concentration of two different solutes‚ bromophenol blue and methyl orange in a mixture. Materials and Methods: Refer to Biological Science practical manual page 5-11. Results: Part 1: Determination of Amax of bromophenol blue The wavelength
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The purpose of this lab was to use a spectrophotometer to find the concentrations of glucose in varying samples of Gatorade. The varying executions included changing the temperature of reaction (room temperature instead of 40 degrees centigrade)‚ changing the time for reaction (20 minutes in the water instead of 45 minutes)‚ and changing the amount of enzyme added (0.1mL of enzyme. The results in from this lab gave data points with respect to ferricyanide. Fortunately‚ ferricyancide and glucose have
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Technical Specification Sheet For UV-Spectrophotometer | | Wavelength range | 190 to 1‚100 nm | Spectral bandwidth | 1 nm (190 to 1‚100 nm)/ variable Low stray light < 0.12%T (220 nm‚ NaI 340nm‚ NaNO2>2.0 Abs) | Wavelength accuracy | ± 0.1 nm at D2 peak 656.1 nm‚ | | ± 0.3 nm for entire range | Reproducibility | ±0.1 nm | Resolution | 1 nm more than 6 wave lengths | Photometric system | Double beam optics | Photometric range | Absorbance: -4 to 4 Abs | | Transmittance:
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mind that all the tubes that would enter the spectrophotometer are clean and dry before the substrate and buffer was added. In the first experiment‚ optimal temperature for enzymatic activity was tested. Five clean spectrophotometer tubes wereare necessary with the different temperatures labeled on them using a wax pencil (Blank‚ Room Temp‚ 35 degrees Celsius‚ 45 degrees Celsius‚ and 55 degrees Celsius). Then 1mL of
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: Joanne Wong Student ID : 00000012636 (BM1/14) Title : Spectrophotometer and its function Introduction Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter‚ UV spectrometer‚ IR spectrometer‚ atomic spectrometer
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Group No. 10 Date Started: June 11‚ 2013 Tongco‚ Val Justin DM. Date Finished: June 18‚ 2013 Santos‚ Erickson S. Vasquez‚ Jona Candace P. Wong‚ Victor Lorenzo E. Experiment 1 Use of Micropipettor and Spectrophotometer Introduction In scientific experiments‚ correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the
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Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A spectrophotometer can be used quantitatively
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Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together
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Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a &
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