then be determined. Purpose: The purpose of this experiment is to determine a value for the equilibrium constant for the reaction between iron (III) nitrate and potassium thiocyanate. Equipment/Materials: Spec 20 or similar spectrophotometer 0.00200 M KSCN 0.200 M Fe(NO3)3 0.00200 M Fe(NO3)3 0.05 M HNO3 burets or pipets 50 mL beakers cuvets Safety: • An apron and goggles should always be worn in the lab. Procedure: 1. The chart below provides the volumes
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of the light. This occurs the most at the analytical wavelength‚ where the most light is absorbed by the particles. When light of this wavelength hits the particles‚ it is absorbed and there is no chance that it will reach the sensor in the spectrophotometer. However‚ if a solution is analyzed with a wavelength corresponding to the color of the particles‚
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Relevance of Chemistry 3 Analytical Chemistry 5 Chemical and Biology Safety 9 Classification of Hazardous Chemicals 15 Biochemistry 24 Organic Reactions 32 Radiation 34 Environmental Concerns 40 The Relevance of chemistry to Occupational Safety‚ Health an Environment Chemistry is the science of chemicals which studies the laws governing their formation‚ combination and behaviour under various conditions‚ it
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CHAPTER I The Problem and its Background Introduction According to Davis (2006)‚ She defined leadership as the ability to persuade others to seek defined objective enthusiastically .it is the human factor which binds a group together and motivate it toward goal. She also concludes that leadership is one of the essential functions that must be performed by all of the managers. She say that success of all managers largely depend on the capacity to lead their subordinates. Leadership is the process
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1. Define the signs of adverse reactions- iatrogenic and idiosyncratic reactions *Adverse reactions- any unexpected‚ unintended or excessive response to a medication given at therapeutic dosages (opposed to an overdose) *Latrogenic- an adverse reaction caused by the actions of a prescriber‚ healthcare professional‚ or other specific treatment *Idiosyncratic- an adverse reaction that is abnormal/unexpected to a medication (other than an allergic) that is peculiar to the individual 2. Steps
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A sample amount of solution protein was made with the respective amounts knowing the concentration of the BSA stock solution‚ and Bradford reagent was added to the samples‚ giving a final volume of 250ul. These solutions were inoculated‚ and in a microplate reader the absorbance was measured at 595nm. A standard curve was created by plotting the absorbance (595nm) vs BSA (ug) data‚ and a best fit line was drawn. Then‚ absorbance readings at 595nm were triplicated using W1-W6 and E1-E6 sample
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Effect of Temperature on Peroxidase Ability to Break Down H2O2 By: Rodneika Crutcher Abstract Temperature affects the ability of peroxidase to break down hydrogen peroxide. In this experiment our professor extracted peroxidase from potato tissue. In order to determine how temperature affects peroxidase we created solutions and measured their absorbance levels after water bath treatments. The more absorbent the solution was the less hydrogen peroxide there was in the solution. This means the peroxidase
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Section M 11 February 5‚ 2014 Absorbance Table Absorbance Spectra Figure 1: Absorption spectra of pigments found‚ through chromatography‚ within Coleus plants. The different wavelengths of light were determined by the use of a spectrophotometer Questions 1. The “total pigment” absorption spectrum provides us with the conclusive amount of light that was absorbed at that specific wavelength. Of which is made up through specific pigments that contribute to a segment of
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Analysis of Quinine in Tonic Water using Fluorescence Introduction: Fluorescence is‚ in a way‚ the opposite of absorption. Rather than recording the light absorbed by a species‚ or the light necessary to excite its electrons‚ we measure the light emitted after the species has been excited and returns to its ground electrical state. Two spectra are produced: emission and excitation. Excitation is similar to absorption‚ where emission records the intensity of the radiation emitted by the species
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back. Procedure Materials * Electric hotplate * Pipette / micro pipette * Conical Flask * Laser beam * Magnetic stirrer * Sodium Citrate * Stirring rod | * Wooden Peg * 10 ml Measuring cylinder * Curvette * Spectrophotometer * Gold Solution (HAuCl4) * Heat proof mat |
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