molecule at a particular place called restriction sites. Restriction enzymes also recognize a specific sequence of nucleotides‚ which vary between 4 and 8 nucleotides‚ in particular‚ palindromic. These restriction enzymes were kept in a 50% glycerol buffer that does not freeze at -20 oC. The four available enzymes used for this experiment were EcoRI‚ AvaI‚ HincII‚ and RsaI. The lengths of the DNA fragments from the restriction digests are used to map the relative locations of the four available enzymes
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electric field. Agarose is a polysaccharide purified from seaweed. An agarose gel is created by suspending dry agarose in a buffer solution‚ boiling until the solution becomes clear‚ and then pouring it into a casting tray and allowing it to cool. The result is a flexible gelatin-like slab. During electrophoresis‚ the gel is submersed in a chamber containing a buffer solution and a positive and negative electrode. The DNA to be analyzed is forced through the pores of the gel by the electrical
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As humans‚ it is estimated that we travel upwards to more than fifty different locations per week. Whether it is a business trip or even a quick trip to the grocery store‚ we travel a lot. Though travelling might sound trivial and just a part of our daily lives‚ it is crucial to acknowledge and not overlook the idea of the act of travelling itself. How do we know where the grocery is? Where is Minnesota on a map? Most importantly‚ Where are we? The knowledge and ability to travel to cities
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Topics in C Programming Bob Hain Introduction This document is not intended to be a text on C programming. Because many of you may not have had the opportunity to use or practice C programming‚ we are attempting to provide a brief description of some of the elements of C which you will need in your laboratory work. We will leave out many topics but will try to provide simple‚ although sometimes incomplete‚ explanations of some of the basic elements of C. Why C? The computer industry is changing
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pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer Incubation Cond. Boil‚ Inc. 37 deg.C 37 degrees C 37 degrees C 37 degrees C for 60 minutes IKI Test + - - + Benedict’s Test - ++ - - Chart 1 – Salivary Amylase Digestion of Starch (continued) Tube # 5 6 7 Additives DI Water‚ Maltose Amylase‚ Starch Amylase‚ Starch pH 7.0 buffer pH 2.0 buffer pH 9.0 buffer
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IEEM 517 Just-In-Time LEARNING OBJECTIVES 1 1. 2. 3. Understand the philosophy of Just-In-Time (JIT) Learn the working procedure of JIT Know the differences between the two production-control systems‚ MRP (the push system) and JIT (the pull system) 1 CONTENTS • Motivation • JIT Philosophy • JIT Procedure – Toyota Kanban Systems • MRP vs. JIT • Summary 2 PRODUCTION AND OPERATIONS MANAGEMENT Product development long term Product portifolio Purchasing Supply network design
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Reviewer DataCom: Chapter 1-5 Chapter 1: Intranet- Restricted group on a company and only allows internal employee access. Extranet- Type of network that allows outside vendors special access to limited info in a company. Protocols- Rules of communication. * An identified sender and receiver * An agreed-upon method of communicating * Common language and grammar * Confirmation or acknowledgement requirements Elements of a Network: * Rules or agreements: protocols or how
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Network Security Concepts Name NTC 410 Instructor ’s Name November 1‚ 2005 File security falls into two categories‚ encryption and access. Access to files can mean physical access to a computer with unsecured files or access via user permissions or privileges in the form of access control lists (ACLs) (Strengthen Your Users ’ File Security‚ 2003). The files kept on a server with NTFS storage can be locked to prevent anyone who does not have the correct permissions from opening them. This is
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CYBER CRIME IN ITS VARIOUS FORMS kimmyarora33@gmail.com Abstract: This Paper is a review paper on the topic of Cybercrime and its forms. The paper describes very basic information about cybercrime. It includes introduction to cyber crime‚ its brief history‚ its types along with their brief description‚ today’s scenario regarding cybercrime and an introduction to cyber security as a weapon against cybercrime . The paper is best suited for those who want to understand what cybercrime exactly
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one of DNA isolation‚ the food sample was crushed before Lysis Buffer was added‚ in part to break down some cell walls‚ but also to increase area of the food sample being touched by the Lysis Buffer. The purpose of Lysis Buffer is to break down the cells in the food sample and release their DNA into the solution. By increasing the surface area to volume ratio of the food sample‚ this process is accelerated. After the initial Lysis Buffer addition‚ the mixture was further ground to increase the contact
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