EXPERIMENT 2: COMMON-ION EFFECT AND BUFFERS MARVILE REA R. FERRER1 1DEPARTMENT OF MINING‚ METALLURGICAL & MATERIALS ENGINEERING‚ COLLEGE OF ENGINEERING UNIVERSITY OF THE PHILIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: DECEMBER 13‚ 2012 DATE PERFORMED: DECEMBER 07‚ 2012 ______________________________________________________________________________________________________________ INTRODUCTION Monitoring the pH range of a laboratory reaction or a process is very
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Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic acid‚ Gylcine
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Conclusion Both Kool Aid and Gatorade contain buffers. For the buffer in Kool Aid‚ pKa1 ≈ 3.70‚ pKa2 ≈ 4.90‚ pKa3 ≈ 6.50 and for the buffer in Gatorade‚ pKa1 ≈ 4.50‚ pKa2 ≈ 5.40‚ pKa3 ≈ 6.70. Discussion of Theory A buffer capacity is the maximum amount of hyrdrogen or hydroxide ion that can be added to a buffered solution before a significant change in the pH occurs. Increasing the concentration of the buffer can increase the buffer capacity of a solution. At the half equivalence point‚ the moles of
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Using Buffers Gino A. Romeo‚ Jr.‚ Ph.D. Version 42-0134-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
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t Design and preparation of buffers effective at different pHs Abstract These experiments aimed to determine the optimum pH ranges various buffers are effective and provide opportunity for the use of the Henderson-Hasselbalch equation to prepare a buffer of a specific pH. Three different buffer systems were initially investigated; volumes of weak acid and weak bases of specified concentration were prepared and titrated against strong acid or strong base solutions with pH readings taken at frequent
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microcentrifuge tubes Test plate Micropipetters and tips DI water Buffer solution … 4.5 to 8.8 I2Kl (grams iodine) Starch solution Enzyme (amylase) 80 degree Celsius water (HOT) Floating test rack Procedure: While controlling the amount of starch and the amount of buffer we use with a pH of 5.8‚ we want to investigate how changes in enzyme concentration affect reaction rates. First we put 500 ml of amylase from 0.2% solution (provided by Michael Bunch) into an experimental tube. We then
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1) Natural buffers are chemicals that the body releases into the blood stream to help maintain a healthy pH level. Carbon dioxide (CO2) acts as an acid by donating hydrogen ions when needed and forms carbonic acid when it dissolves in water. Carbonic acid bicarbonate is important for maintaining an acid base balance in the blood as it equalizes the pH (7.5) of the blood. All body fluids have buffers that defend the body against pH changes. A process that affects buffers in the blood is exercise
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A buffer solution can maintains a narrow range of pH even when strong acid are added. In our experiment‚ we can see water is not a buffer‚ water can undergo very big changes when small amounts of strong acid or strong bases are added. When the strong acid‚ 100µl HCl was added into the dd water‚ the pH value changed from 7.38 to 5.83 which mean the dd water has turn into acid. The same phenomenon occurs while strong base‚ 100µl NaOH was added into the dd water. The pH value changed from 7.07 to 9
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9001:2008 Certified Institute (Indian Standard Organization(ISO) Certificate Number:2071-QMS-1071) Email id: hsbsolutions7@gmail.com website:www.hsbsolutions.jimdo.com Ph: 9210846949 / 9717925135 Physics: Chapter 2 Solutions Last 15 years questions with solutions Topic: Strength of Solution Q.1Discuss the effect of Temperature on Solubility of solids in Solvent. AnsThe solubility of solid in a liquid solvent generally increases with increase in temperature of the solvent. Very rarely some neutral
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determine the type of substance with comprise buffer solutions and identify buffer solutions. The significance of the study is to know the effect of adding strong acids or bases to buffer solutions especially that of the body. Part A determines the effect of common ions to the extent to ionization wherein certain reagents were mixed with water in one test tube and a solution with a common ion in another. Part B determines which solutions exhibit buffer effect wherein certain reagents were mixed and
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