Question 1 Critique Dr. Honeydew’s report. Specifically‚ discuss what is missing and how the missing information should have been presented. First the PH measurements‚ Dr. honey dew does not explain how the PH device was calibrated or if the blood sample was added or mixed with anything prior to the measuring of the pH (like water‚ etc.). In regards to the absorption spectroscopy‚ the method by which the concentration was obtained is questionable. Was the concentration known before the experiment
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that increasing pH levels will increase the rate of amylase activity; pH levels at 7 or higher will show a fast rate of amylase activity. Variables: Independent Variable: The independent variable was the varied pH levels manipulated by using a buffer solution of different pH levels. The different pH levels were pH0‚ pH4‚ pH6‚ pH8 and pH10 Dependent Variable: The dependent variable is the amylase activity. Amylase activity was measured by recording the concentration of starch in each test tube
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due to the decrease of NADH concentration in unit time is proportional to the urea concentration. Ammonia which is produced by different decomposition processes is also determined by this method. REAGENTS COMPOSITION 5 volume of Reagent 1 contains buffer and was mixed with 1volume of enzyme reagent (R2). Working reagent were prepared fresh prior to use.
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constructed the following year. James Oglethorpe‚ a hardy solider‚ became the commander of the colony after being appointed by the King. Georgia was unique to the English colonies because of the area it resided in. Georgia was used as a military buffer that would provide protection to South Carolina from the Spanish. This was deemed necessary after the Spanish had threatened Charleston on multiple occasions. In order to provide success for the colony‚ Oglethorpe tried to keep the colony under better
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The materials needed for this experiment were four medium sized tubes‚ a spectrophotometer‚ a buffer with a pH of 5‚ H2O2‚ Peroxidase‚ and Guaiacol Dye. We as a group had four tubes labeled two‚ three‚ four‚ and five. In the tube labeled two we had a solution of 2.0 mL of H2O2 and 1.0 mL of Guaiacol Dye for a total solution of 3.0 mL. In the tube labeled three we had a solution of 4.0 mL buffer and 1.0 mL Peroxidase for a total of 5.0 mL solution. In the tube labeled four we had a solution of
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SCHOOL OF TECHNOLOGICAL SCIENCES INDUSTRIAL ENGINEERING DEPARTMENT Simulation Project- IE532 * Table of Contents: 1. Introduction……………………………………………………………………………………………………..2 2. Approach………………………………………………………………………………………………………….3 3. Input Analyzer………………………………………………………………………………………………….9 4. Animation……………………………………………………………………………………………………….10 5. Results…………………………………………………………………………………………………………….11 4. Discussion……………………………………………………………………………………………………….15 5. Conclusion………………………………………………………………………………………………………16
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Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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CHEM RESEARCH TASK 1. INDICATORS Identify and describe some everyday uses of indicators including the testing of soil acidity/basicity. • Indicators are used regularly in chemical laboratories during chemical reactions. One important use is to determine the end point of a titration when an acid reacts with an alkali. The reactants and products of such a reaction are colourless so an indicator is a useful way to visually determine when the reaction is complete. Chemists also need to monitor
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The .75 agarose gel is used so scientists can see when DNA runs through it. They mixed 45 mL of the TAE buffer with .75 grams of agarose powder. The group used TAE buffer rather than water because the TAE buffer is a salt based liquid. It contains a mixture of Tris base‚ acetic acid and EDTA. This means it is positively charged‚ and when electricity is run‚ the TAE buffer guides the electricity from negative to positive‚ which will be crucial for a future step. This happens because two
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Preparation and storage of buffers and general solutions used: All Solutions were made with Ultra-pure water (MilliQ water). The 4% formaldehyde solution was freshly made in PBS. (phosphate buffer saline). A 0.1 % solution of Triton X-100 was made prior to use in PBS. The t-RNA solution is made in water at 10 mg/ml and stored in aliquots at −20 °C. The 20× SCC:
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