supplied food acid is citric acid (triprotic) or tartaric acid (diprotic acid) Materials 4 x 100mL conical flasks 50mL burette rubber bulb 1 x 200mL beaker white tile retort stand 20mL volumetric pipette volumetric flask stopper 300mL distilled water marker 40mL 0.3M food acid burette clamp 0.1M sodium hydroxide magnetic flea magnetic stirrer glass funnel 1 x 200mL volumetric flask phenolphthalein 4 x 50mL beakers Method Part A: Making the food acid Place distilled
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Lloyd Term 2‚ 2014 Lauric Acid Experiment Teacher: Mr Oliver Contents 1.0 Introduction Elements and the compounds can exist in distinctive physical states. Liquid‚ solid‚ gas and plasma. Every element and compound has a distinctive melting and boiling point. When a substance reaches its melting and boiling point it will change phase. For example the molecules in a solid being heated gain energy and eventually are able to not only vibrate but
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The type of acid was clearly controlled in the method. It clearly states that hydrochloric acid is representing the digestive chemical whilst the antacid tablets represent the food being digested. It is evident that the hydrochloric acid was the acid used in all aspects of this experiment successfully controlling this variable. It is not certain whether the amount of acid was controlled throughout the experiment. This is because the amount of acid was not clearly outlined neither
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Structures‚ Biosynthesis and Biofunctions of Iron-sulfer proteins Yiming Chen‚ Brown University‚ May 11th‚ 2011 I. Introduction Iron-sulfur proteins are the proteins which contain iron-sulfur clusters‚ like sulfide-linked di-‚ tri-‚ and tetrairon centers with various oxidative states 1. An excess of 120 distinct types of enzymes and proteins are known to contain Fe-S clusters2. Iron-sulfur proteins are known for the role of the oxidation-reduction reactions of mitochondrial electron transportation
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Acids‚ Bases‚ and Buffers Introduction: The pH scale is used to determine how acidic or basic a solution is‚ ranging from 1-14. The most acidic of all acids are at a pH level of 1 and the most basic of all bases are at 14. The neutral pH level is 7‚ which is what drinking water is. The pH level is determined by the amount of H+ ions present in a solution‚ and the more H+ ions there are the more acidic it is‚ and the lack of these ions results in more basic solutions. One distinguishing feature
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4.4. Direct determination of saliva proteins Protein contaminated with nucleic acids absorbed the light at wavelength 280 nm and it absorbs much strongly at wavelength 205 nm when it is free from nucleic acids. The UV-visible spectrophotometer was used in determination of saliva proteins (Figure 2.2). Cold trichloroacetic acid (10 % w/v ) was added to the sample‚ centrifuged for 10 minutes to precipitate protein. The absorbance of a known volume
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Lipids Lipids don’t dissolve in water contains mostly C‚H‚O (lower ratio of oxygen than do carbohydrates Types: Triglycerides‚ Waxes‚ Phospholipids‚ Sterols Triglycerides: contain glycerol molecule with three fatty acids attached‚ glycerol forms backbone different triglycerides are formed by various fatty acids that attach. Types of triglycerides: Saturated Fat‚ Unsaturated Fat‚ Monounsaturated Fat‚ and Polyunsaturated Fat Saturated Fat: all carbons are saturated with hydrogens there
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
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Specificity of Protein-Ligand Binding 1). One can conclude that Orange G. has the lowest affinity for the albumin. In the experiment the concentration of Orange G that binds to protein was a lot less than either Ponceau S. or Bromophenol blue. The ligand with the highest affinity to BSA was a little more difficult to decipher. The experiment shows that all of the 2 µL of both Ponceau S. and Bromophenol blue bind to the BSA. However‚ when 5 µL of the ligand is added the BSA is saturated and cannot
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