Chlorophylls and Beta Carotene from Plant Leaves Wang Haina A0133901R 1. Aim 1. To isolate chlorophyll and beta carotene from plant leaves using column chromatography. 2. To qualitatively characterise the pigments with UV-vis spectroscopy and TLC. 2. Results and discussion Isolation of beta carotene and chlorophylls by column chromatography Upon the loading of S1 (the extract of the plant leaves in hexane)‚ a yellow band appeared at the top part of the silica column immediately after the solvent
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Information and Research 1. Paper chromatography is used to break down colors with using water and the way the colors separate. 2. Doctors can use it to test for alcohol in people’s blood. People can also use it to check for pollutants in the water. Pharmacists can use it to find out how much of each chemical is in each product. 3. I searched it up on google. Purpose The purpose of this test is to see what colors are in certain colors. We use chromatography to separate them/ Materials
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Title : The Vitamin C content of fruit juice Aim : To investigate the vitamin C content of fruit juice. Introduction : [2]Vitamin C‚ also known as the ascorbic acid or the L-ascorbate‚ is required for the growth and repair of tissues in all parts of our body. It is necessary to form collagen‚ an important protein used to make skin‚ scar tissue‚ tendons‚ ligaments‚ and blood vessels. Vitamin C is essential for the healing of wounds as well as for the repair and maintenance of cartilage‚ bones
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Appearance of solution. Dissolve 1.0 g in 9 ml of alcohol R. The solution is clear (2.2.1) and colourless (2.2.2‚ Method II). Related substances. Examine by liquid chromatography (2.2.29). Prepare the solutions immediately before use. Test solution. Dissolve 0.10 g of the substance to be examined in acetonitrile for chromatography R and dilute to 10.0 ml with the same solvent. C. N‚N′-diacetyl-L-cystine‚ Reference solution (a). Dissolve 50.0 mg of salicylic acid R in the mobile phase and dilute
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CHEMISTRY REVISION GUIDE for IGCSE Coordinated Science This revision guide is designed to help you study for the chemistry part of the IGCSE Coordinated Science course. The guide contains everything that the syllabus says you need you need to know‚ and nothing extra. The material that is only covered in the supplementary part of the course (which can be ignored by core candidates) is highlighted in dashed boxes: Some very useful websites to help you further your understanding include: •http://www
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Caffeine is a drug that is used to improve mental alertness by stimulating the central nervous system [1]. It is an ingredient found in a various amount of products sold on the market today‚ such as coffee‚ tea‚ chocolate‚ soft drinks‚ and energy drinks. Caffeine can be taken by mouth or intravenously (by IV) to help alleviate breathing problems in infants and can even be used in combination with some pain killers to treat migraines after epidural anesthesia. Moreover‚ it can be helpful in preventing
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CHAPTER 1 INTRODUCTION Power transformers are very important in the electrical power sector; they are among the expensive assets of power generation and distribution systems. . According to Piush Verma [16]‚ transformers are static electromagnetic devices consisting of two or more windings which are linked with a common magnetic field used for the distribution of electrical power from one voltage level to another. Some of the main parts of the transformer are; the windings‚ tank and insulation
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IDENTIFICATION OF UNKNOWN SOLUTIONS USING TLC CHROMATOGRAPHY Purpose: To become familiar with the principles and terminology of TLC chromatography to identify an unknown molecule based on comparisons with known laboratory standards and Rf factors. Background Thin Layer Chromatography: Chromatography is a sophisticated method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that
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of 95% ethanol producing long red needles. 0.536g‚ 2.07mmol‚ 158-160°C (160°C lit3)‚ 54.6% recovery. Thin layer chromatography was performed in three different eluents‚ hexane‚ toluene‚ and acetone. The chromatography compared aniline‚ 2‚ 4-dinitrobromobenzene‚ and a sample from the mixture of the two reactants when the reaction first began to take place. Another thin layer chromatography used a product sample an hour after the reaction first began and the eluent that showed the best separation of
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LABORATORY REPORT FOR BIO411 Experiment 5 : Cellular Respiration Experiment 6 : Photosynthesis Title: Cellular Respiration Objective: To observe and determine cellular respiration in yeast/onion cells. * Measure respiration rate using different substrates. * Measure respiration rate at different temperature. Introduction: In this laboratory experiment‚ we are given 3 task. The first one is respiration in yeast. Second is respiratory indicator and the third one is observing
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