such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate‚ initial protein concentration was shown to be 100 mg/ml. The final protein concentration of the pooled affinity sample
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Beam Chopping corrects for flame emission but not for scattering. Can distinguish the signal of the flame from the atomic line at the same wavelength. The higher the absorbance the higher the transmittance. Intro to IR Near IR- (0.78 to 2.8 micrometers) Mid IR- (2.5- 50 micrometers) Far IR- (50-1000 micrometers) Most common region used is Mid IR btwn 2.5- 15 micrometers Mid IR: based on diffracting gratings but now current instrumentation are of Fourier transform type Far-IR: useful
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extraction with 10 mM formic acid solution and precipitation at low temperature by centrifugation. The utilise of C-18 reversed-phase columns were slightly more polar performed to achieve a better separation of acrylamide. Eluent system of liquid chromatography used a time programme gradient in order to obtain a better selectivity. The mobile phase was a mixture of 10 mM formic acid and methanol. The performance parameters of precision‚ accuracy and recovery were conducted to assure that the analytical
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The five common methods that can be used to separate various types of mixtures are filtration‚ fractional distillation‚ evaporation‚ sublimation‚ and chromatography. Evaporation is the changing of a liquid into a gas often under the influence of heat. A mixture of a solid and liquid can be separated by evaporation. Filtration is a technique that uses a porous barrier to separate a solid from a liquid. A liquid
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Mixtures & Solutions Lab Report By Nathan Mitchell 4/23/15 Background Information & Research 1. Paper Chromatography is a method used for the separation of colors which are also referred to as colored chemicals/substances or pigments. This method is used for experiments‚ to identify coloring agents and to separate out a compound into its various components. 2. 3 real-world uses for paper chromatography include forensic testing‚ performance enhancing drug testing‚ and Ebola immunization. 3. References:
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Colorado Northwestern Community College Science of Biology Mrs. Farrow Lab 3 – Slime Time Submitted by Chase Kenemer 22 February 2015 Abstract Polar solvents dissolve‚ or pick-up‚ polar substances and non-polar solvents dissolve‚ or pick-up‚ non-polar substances. In the conducted experiment‚ the polarity of molecules and their properties are explored. The results of using two solvents on both polar and non-polar inks‚ further verify this to be true. The student conducted the experiment
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solution contains compound A with 4 polar groups and compound B with 2 polar groups. You plan to separate these compounds using paper chromatography with a non-polar solvent. Predict the location of the two bands relative to the solvent front. Explain your answer. Since compound A is probably more polar than compound B‚ compound B will move further in the chromatography. Since the solvent is non-polar‚ the less polar compound will be more soluble in the solvent than the more polar one. Applying Your
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which is glycerol is filtered off by a separation process and an impure biodiesel will be obtained. The biodiesel obtained is still contained a little amount of glycerol and other impurities. Hence‚ to get pure biodiesel‚ high-performance liquid chromatography (HLPC) is used to further purify the biodiesel. The biodiesel yield can be calculated relative to the initial amount of Pongomia Pinnata oil by weight and the biodiesel purity is determined
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The E2 reaction performed began by dehydrohalogenating the 3-chloro-3‚ 7-dimethyloctane with potassium hydroxide in aqueous ethanol. The 3-chloro-3‚ 7-dimethyloctane will lose a hydrogen from three different carbons that cause the formation of three constitutional isomers. If the hydroxide ion attacks a hydrogen on the 3-methyl carbon‚ then 2-ethyl-6-methyl-1-heptene will be the product. If a hydrogen on the carbon-2 was attacked‚ then the product would be 3‚ 7-dimethyl-2-octene. If a hydrogen was
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for epimers and isobars Iltaf Shah1‚ Ricky James1‚ James Barker2‚ Andrea Petroczi1 and Declan P Naughton1* Abstract Background: Recently‚ the accuracies of many commercially available immunoassays for Vitamin D have been questioned. Liquid chromatography tandem mass spectrometry (LC- MS/MS) has been shown to facilitate accurate separation and quantification of the major circulating metabolite 25-hydroxyvitamin-D3 (25OHD3) and 25hydroxyvitamin-D2 (25OHD2) collectively termed as 25OHD. However
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