temperature of water will be increased in increments of 10oC‚ thus the temperatures I will be using are 20oC‚ 30oC‚ 40oC‚ 50oC and 60oC. Dependent Variable: I will be measuring the cell membrane permeability through the amount of pigment dispersion. A colorimeter will be used to quantify results into a percentage transmission of light‚ after it is left for 5 minutes. Control Variables: Variable How it is measured Why it needs to be measured Mass of beetroot cylinder A weighing scale should be used to ensure
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Why does belatin leak out of cooked Beetroot? In cooked beetroot‚ the water or food would become stained due to its leakage of betalin (the redish-purple pigment). Betalin is found in the vacuole of beetroot cell. The leakage may have been caused by something happening in the cell membrane of the beetroot – concerning the proteins embedded in the phospholipid layer. The study will require the experiment of different temperatures of water and to find out which in temperature causes the most leakage
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in sulphuric acid to create the blue solution of copper. We put the blue solution of copper in the colorimeter and measured the absorbance of the solution 3 times then we found the average of the 3 different results we had gotten. We then diluted the blue solution of copper with water and put it into the colorimeter and measured the absorbance 3 times again and got the average again. A colorimeter is a light-sensitive instrument that measures how much colour is absorbed by an object or substance
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conical flask beside windows. It will eventually influence the reliability of the lab‚ since increase in temperature will also increase the rate of respiration until a certain optimum temperature. Same Colorimeter device Using the same colorimeter device throughout the experiment. Different colorimeters Collaborates differently which might outcome in functions or changes in percentage Using the same cuvette throughout the experiment. Make sure to rinse the cuvette after use‚ in order to use it again
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very similar structure to chymotrypsin; the Trypsin we used was from a beefs pancreas. Apparatus used in this investigation 1. Acidified protease solution 2. Milk powder 3. Standard glass wear 4. Stop Clock 5. Cuvette 6. Colorimeter 7. Measuring cylinder Before making my hypotheses I investigated what affects rates of reactions and the enzyme structure. The specific shape of the protein determines the active site for specific reactions‚ which brings me onto the lock
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violet while it reacts with sodium hydroxide with respect to crystal violet. The amount of sodium hydroxide is varied in this experiment while crystal violet is kept at a constant. The transmittance of crystal violet is observed and recorded using a colorimeter and the data obtained is used to plot graphs which are manipulated using LoggerPro software to produce the desired outcome; rate of reaction of crystal violet. Upon completion of the experiment it was seen that the rate of reaction of crystal violet
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purposely made 1000 times larger then Concentration of CV+. Thus‚ the concentration of OH- changes so little during the reaction that can be assumed to be constant. Materials: IBM Compatible computer‚ serial box interface‚ LoggerPro Software‚ Vernier colorimeter‚ plastic cuvette‚ 250-mL beaker‚ 0.020M NaOH‚ 2 x 10-5 M crystal violet‚ distilled water‚ stirring rod‚ two 10-mL graduated cylinders. Procedure: 1. Obtain and wear safety glasses. 2. Measure out 10 mL of 0.020M NaOH using a 10 mL graduated
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equipment and apparatus used in this experiment: ➢ Colorimeter ➢ Thermometers ➢ Water baths at various temperatures ➢ Stopclock ➢ Test tubes ➢ Small measuring cylinders ➢ Cuvettes ➢ Cork borer ➢ Mounted needles ➢ Beaker about 250 cm³ ➢ Blue Filter The dependent variable was the absorbance. We measured it by using a colorimeter which compares the amount of light getting through a solution
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INTRODUCTION Chemical kinetics‚ which can also be explained as reaction kinetics‚ studies the rates of chemical processes and reactions. A lot of this has to do with the speed at which a chemical reaction occurs for instance the rate at which reactants and products appear and disappear. In this case chemical kinetics goes further in depth to understand what conditions affect the rate of a given reaction. This is important because it allows scientists to determine what type of reaction can occur
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plasma membrane. Overview The beetroot discs will be placed in distilled water at different temperatures. You will compare the intensity of the beetroot pigment that has leaked out from the cells subjectively and possibly objectively using a colorimeter. You can relate the findings to the effect of different temperatures on the permeability of cell membranes in beetroot (as well as in other organisms). Features of experimental design The investigation contains these main features of experimental
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