this experiment‚ a mixture of two compounds‚ cyclohexane and toluene‚ was separated into fractions by the techniques of simple and fractional distillation. The individual fractions that were gathered from the distillation were analyzed using gas chromatography (GC) and used to compare the efficiencies of the two different distillation techniques. The ultimate goal of this experiment was to determine whether simple or fractional distillation was the more efficient means of separating volatile compounds
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Yuri Anthony D. Sucgang BMLS-IA Electrophoresis Machine Gel Electrophoresis Apparatus(Aragose Gel) Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. Gel electrophoresis is a laboratory procedure used to separate biological molecules with an electrical current. In this lesson‚ we’ll review how agarose gel electrophoresis works and introduce the equipment necessary to perform an electrophoresis experiment.
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It is well recognized now that column band broadening originates from three main sources: 1. multiple path of an analyte through the column packing; 2. molecular diffusion; 3. effect of mass transfer between phases. In 1956 J.J. Van Deemter introduced the equation which combined all three sources and represented them as the dependence of the theoretical plate height (HETP) on the mobile phase linear velocity. Originally‚ it was introduced for gas chromatography‚ but it happened that the same
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lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of two or more substances between a stationary
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for epimers and isobars Iltaf Shah1‚ Ricky James1‚ James Barker2‚ Andrea Petroczi1 and Declan P Naughton1* Abstract Background: Recently‚ the accuracies of many commercially available immunoassays for Vitamin D have been questioned. Liquid chromatography tandem mass spectrometry (LC- MS/MS) has been shown to facilitate accurate separation and quantification of the major circulating metabolite 25-hydroxyvitamin-D3 (25OHD3) and 25hydroxyvitamin-D2 (25OHD2) collectively termed as 25OHD. However
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experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to observe the effect of the increasing polar eluent fractions‚ to analyze the different fractions collected‚ and to correlate the structure of the components with polarity and behavior on a chromatographic column. Background The best method for separating mixtures in organic chemistry is chromatography. Chromatography is the separated of a mixture of two
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References: Experimental Organic Chemistry: A Miniscale and Microscale Approach‚ 5th ed. By Gilbert and Martin‚ Section(s)2.13- 2.14‚ 4.3-4.4. Investigating gas chromatography. (2013). Retrieved from http://www.vernier.com/experiments/chem-o/8/investigating_gas_chromatography/ Figure 8: Pre Fractional Distillation GC Results Sample | Boiling range (°C) | Volume (mL) | % Composition (lower bp : higher bp) |
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process heating a liquid to form vapor which is then collected when cooled and separated from the original liquid. The purpose of this experiment was to determine the identity of an unknown substance using simple and fractional distillation. Gas chromatography analysis the components of a mixture by separation of the materials by their different boiling points. EXPERIMENTAL The simple distillation apparatus was assembled using a 10-mL round bottom flask‚ distillation head‚ thermometer‚ adapter‚ thermometer
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Today in lab I will separate mixtures of compounds into their constituent components using chromatography paper and an eluting solvent of salt water as well as rubbing alcohol. Materials and Methods: In this lab using the bag of FD&C dyes‚ bag of M&M’s‚ 2 packs of Kool-Aid ‚ and a pack of Clover Valley store brand food coloring I am going to place a drop of each dye at the bottom of the chromatography paper. For the experiment with the rubbing alcohol I also placed a drop of each dye‚ but used
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The BSA is about 66‚000 Da‚ and the phenol red is about 350 Da. The free phenol red will elute last due to its smaller size (350 Da). Small molecules easily enter and fit in the many pores of the beads. Therefore‚ it takes more time to run down the column and then elute
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