Proteins (ACTIVITY 8) Gallemit‚ Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and
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Identification of Plant Pigments by Paper Chromatography Biology 1107L Introduction All living organisms require energy for their chemical processes. The ultimate source of this energy is the sun. Plants convert light energy into the chemical energy of sugars. During photosynthesis pigments are used to capture light energy. Pigments of green plants can easily be separated and identified using a technique called paper chromatography. The purpose of this experiment is to separate
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Bhindia Pooja Rayel Title : Paper Chromatography Photochemical Smog Lecturer’s Name : Ms. Usha Nanthini Table of Content No. | Content | page | 1. | Paper Chromatography | | | 1.1 Definition | | | 1.2 Background | | | 1.3 Rf Values | | | 1.4 Principles of Paper Chromatography | | | 1.5 Procedure of Paper Chromatography | | | 1.6 Uses of Paper Chromatography | | | 1.7 Application of Paper Chromatography | | 2. | Photochemical Smog | | | 2.1
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Procedure The procedure stated in Chem 2120 experiment 6 Williamson Ether Synthesis of Phenacetin laboratory manual was followed without any major changes. Data and results Compound Amount used MW (g/mol) Moles Stoichiometry/Comments acetaminophen 0.354 g 151.16 2.34 x 10-3 limiting reagent ethyl iodide 0.3mL 155.97 3.75 x 10-3 1.6 equiv ’s sodium ethoxide 2.6mL 68.05 3.3 x 10-2 catalyst‚ reaction solvent crude product obtained: phenacetin 0.32g
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EXTRACTION OF XANTHONE IN GARCINIA MANGOSTANA L. (MANGOSTEEN) FOR TOOTHPASTE BY : BACHELOR OF SCIENCE (CHEMISTRY SCIENCE) CHAPTER 1 1.1 INTRODUCTION 1.2.1 Introduction Mangosteen is the common name for Garcinia mangostana L. (GML). The fruit is dark purple or reddish in colour‚ with white‚ soft‚ and juicy edible pulp. The fruit taste is sweet with a slightly acid and also have a pleasant aroma. Nowadays‚ there were a lot of studies and researches about the extraction
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PROCEDURE Part I Setting Up Experiment 2D: Separation of a mixture by Paper Chromatography Objectives: 1. To assemble and operate a paper chromatography apparatus 2. To study and the significance of Rf values 3. To test various food colorings and to calculate their Rf values 4. To compare measured Rf values with standard Rf values 5. To separate mixtures of food coloring into their components 6. To identify the components of mixtures by means of their Rf values Materials / Apparatus: See “Health
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pH and Chromatography Lab Report Kevin Rivera Biology Mr. Langley 2C 10/7/13 Introduction In this lab‚ of pH and Chromatography‚ in the pH aspect of the lab we are trying to figure out the pH level of certain chemicals by writing down of known solutions to find the type of unkown solution using pH standards. In the Chromatography‚ we are trying to separate the chemicals using water and a piece of paper. Methodology Materials: Safety Goggles pH indicator & pH indicator key
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the sample. Uses for Chromatography: *Pharmaceutical company - can determine how much chemical is in a new product or food. *Hospital- Can detect blood or alcohol levels in a patient’s blood. *Law enforcement- Can compare a sample found at a crime from the suspect. *Environment agency- Can determine the levels of pollutant in the water supply. *Manufacturing Plant- Can purify a chemical needed to make a product. Chromatography is very important
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Jacobsen’s Method of Epoxidation of an Alkene | | Abstract Various types of reactions were completed to first create and then use Jacobsen’s catalyst in the asymmetric epoxidation of an unknown alkene with bleach in the laboratory. The chiral epoxide synthesized was then characterized with GC/MS and NMR. With this information the unknown alkene was able to be identified as 4-chlorostyrene. Introduction Organisms have evolved with mechanisms that use specific enantiomers of molecules
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is equal * protein aggregates since the electrostatic repulsive forces are at a minimum. 4. Precipitation with organic solvents or acidic protein precipitants -addition of O.S decreases dielectric constant PURiFICATION 1. Column chromatography 2. Density gradient centrifugation 3. Electrophoresis 4. enzymatic digestion 5. Removal of small ions or molecules or to exchange one ionic environment for another during purification is done by : Dialysis‚ Ultrafiltration
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