nature of agarose‚ a solution of agarose can be heated and cooled to form a gel in a casting tray. Think of casting the agarose gel like pouring hot gelatin into a mold. The hot agarose liquid is poured into a casting tray. Once the mixture cools‚ a thin agarose brick will form. To ensure there’s a place to put the DNA in the gel‚ a comb is placed in the agarose liquid before it cools. Each tooth in the comb will become a hole‚ or ’well‚’ in the solidified agarose gel. Once cast‚ this gel is placed
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solid support‚ silica gel‚ which acts as a Lewis acid catalyst to facilitate the reaction. The reaction forms a porphyrinogen‚ which is then oxidized to the porphyrin product by atmospheric oxygen. Column chromatography is performed for the isolation and purification of tetraphenlyporphin‚ and the thin layer chromatography for analysis.It was concluded that the renention factor(Rf) of the 5‚10‚15‚20- Tetraphenylporphyrin with a percent yield of 61% Introduction: In this experiment 5‚10‚15‚20‚ tetraphenylporphyrin
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CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
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+ NaI + H2O CH3I Before coming to lab‚ please review the following techniques: "Reluxing a reaction‚" "Extraction and washing‚" "Drying an Organic Solvent‚" "Evaporating an Organic Solvent‚" "Analyzing a Solution by Thin Layer Chromatography (TLC)‚" "Separating Mixtures by Column Chromatography‚" and "Characterizing Compounds by IR." Notice that this lab brings together most of the techniques that we have used this semester. Then complete the following table before coming to class so that you will
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EXPERIMENT 15 Thin-Layer Chromatographic Analysis of Drug Components Experiment Objective: To identify the components of an analgesic drug tablet and then correctly identify the tablet from a group of others with acquired data. Experiment Summary: In this experiment‚ we use TLC to identify components of an unknown analgesic drug. We prepare a solution of the drug by dissolving part of a tablet in 1:1 ethanol/dichloromethane‚ then spotting a TLC plate with the solution along
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3. This is due to the structure of disperse red 9 being more symmetrical than that of disperse blue 3 and having more nonpolar bonds. Disperse blue 3 is more polar because it has a hydroxide bond and has a larger dipole. The principle behind using column chromatography is that it separates compounds based on polarity. The alumina serves to allow for a purer separation than TLC plates because it has a more polar surface than silica gel does. The less polar dye moves first because it is not as soluble
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contents; thin epidermal cells; lignified narrow spiral and annular vessels; scattered starch grains; rhomboid crystals of calcium oxalate; sclereids of the epidermis and dry scales. Identication (Thin Layer Chromatographic Identification Test for Crude Plant Drugs) – Test solution—Transfer 1.0g of dried allii sativi Bulbus Pulveratus to a suitable glass apparatus. Extract with 10mL of a mixture of methanol and water (95:5)‚ using any suitable method as described in Test Solution under Thin Layer Chromatographic
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Column Chromatography ________________________________________________ You have already performed two chromatography experiments: gas chromatography and thin layer chromatography. All chromatography experiments involve passing a mixture of analytes through a system that includes a mobile phase and a stationary phase. The partitioning of the analytes between these two phases determines the rate at which they pass through the system‚ and (in theory) allows them to be separated from one another. Column
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liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together with a standard caffeine. The Rf value obtained for both isolated caffeine and standard caffeine was 0.24. Keywords: solid-liquid extraction‚ multiple liquid-liquid extraction‚ sublimation‚ thin layer chromatography‚ percent caffeine Introduction Many organic compounds are obtained from natural sources through isolation
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Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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