Introduction: The purpose of this experiment is to use sodium borohydride to reduce benzil. However‚ stereochemistry allows for five possible products. If only one carbonyl group is reduced during the reaction a racemic mixture of benzoin will be the product that is produced. After the first reduction a chiral center forms causing the second reduction to occur from only one side of the ketone. Depending on which side the second reduction take place there are three possible products including: a racemic
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Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography
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Current Regulations for Standardization of Crude Drugs In India a great deal of bulk knowledge exists among ordinary people about the traditional use of herbal medicine. It is difficult to quantify the market size of the traditional Indian system. Since most practitioners formulate and dispense their own recipes. The present annual turnover of product manufactured by large companies is estimated at approximately US $ 300 million compared to a turnover of approximately US $ 2.5 billions for modern
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These peaks show that the solution in vial 4 contained dichloromethane. This is viable because the column did contain dichloromethane‚ which is what the original 0.70 mL of the bottom layer solution was filtered through. Also the .7 mL of the solution that was ran through the column was a dried dichloromethane solution. The peaks that represented 2-naphthol‚ 1630 cm-1‚ 1364 cm-1‚ and 1216 cm-1‚ also showed that the solution in vial 4 contained
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using simple distillation. The purity of the product will be characterized using percent yield‚ thin layer chromatography‚ Beer’s Law‚ and UV-vis. Beer’s law is used because in this experiment we are comparing our extraction of B-Carotene to the Stock B-Carotene. Beer’s law shows that absorbence of light at a specific wavelength is proportional to the concentration of the colored species in the solution. Thin layerChromatography is used to separate mixtures of substances into their component‚ thus allowing
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the presence of 95% ethanol producing long red needles. 0.536g‚ 2.07mmol‚ 158-160°C (160°C lit3)‚ 54.6% recovery. Thin layer chromatography was performed in three different eluents‚ hexane‚ toluene‚ and acetone. The chromatography compared aniline‚ 2‚ 4-dinitrobromobenzene‚ and a sample from the mixture of the two reactants when the reaction first began to take place. Another thin layer chromatography used a product sample an hour after the reaction first began and the eluent that showed the best separation
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There can be both advantages and disadvantages in using a TLC sheet of a different length. For example‚ it becomes a disadvantage if the TLC sheet in this lab was 5 cm shorter. If this were the case‚ then (Sample A) would not have shown up or the second spot of unknown substance #3. Moreover‚ the unknown substance‚ which consists of (Sample A‚ would be incorrectly identified as a pure substance and/or as consisting of only (Sample D). Contrary to using a shorter TLC sheet‚ a longer TLC sheet might
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SCIENCE SBT 4534 ANALYTICAL METHODS FOR FUNCTIONAL FOODS & NUTRACEUTICALS LABORATORY REPORT LAB 2: FAT & OIL FROM OIL-SEEDS: PART II THIN LAYER CHROMATOGRAPHY ANALYSIS (TLC) COORDINATORS: ASSOC. PROF. DR. JAMALUDDIN BIN MOHD DAUD ABDUL AZIM ADNAN 1016741 INTRODUCTION TLC chromatography or thin layer chromatography is a type of planar chromatography. TLC is routinely used by researcher in the field of phyto-chemicals‚ biochemistry etc. to identify
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Briefing Thin-layer chromatography (TLC) is an extremely valuable analytical technique. It provides a rapid separation of compounds‚ and gives an indication of the number and nature of the components of a mixture. TLC can also be used to identify compounds by comparison with known samples‚ to check the purity of a compound‚ or to monitor the progress of a reaction‚ an extraction‚ or a purification procedure. This experiment will introduce you to the mechanics of TLC‚ and the chemical principles
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discussed in the lecture course‚ and to familiarize you with the principles employed in identifying a simple organic compound. Occupational Health and Safety Notices The Elements 1. Nitration of acetanilide 2. Hydrolysis of p – nitroacetanilide and thin layer chromatography 3. Separation of a three – component mixture by extraction 4. Completion of experiments from day 1 to 4 5. NMR workshop 6. Sandmeyer Reaction: Preparation of p-chloronitrobenzene 7. Synthesis of benzilic acid from
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