of the pure hexane and one mL of the pure acetone to the mortar. Grind the contents until the cell walls have been adequately broken. Transfer the mixture to a centrifuge tube to separate the sample into layers‚ to help remove the water. Once the sample is finished spinning extract the organic layer and place in a test tube. TLC: To determine which mixture acetone and hexane is the best developing solvent the first step is to make mixtures of these solvents in test tubes. In the first test tube
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1. In table format‚ provide the Rf values you recorded for each of the 3 compounds in Part I. List compound on one axis of your table and solvent system on the other. If multiple spots were present for a compound‚ give Rf value for each component and state if the component was major or minor. | 100% hexane | 25% EtOAc in hexane | 10% EtOAc in hexane | Fluorene | 0.34 cm | 0.94 cm | 0.67 cm | 9 - fluorenol | 0.00 cm | 0.49 cm | 0.05 cm | 9 - fluorenone | 0.00 cm | 0.69 cm | 0.21 cm |
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I. INTRODUCTION A. Background of study Candy-coated sweets come in different colors and sometimes even vary in flavor in association with the colors. These colors provide a creative display for children to easily get attracted to the particular treat‚ leaving those tinted marks on their mouths and a happy‚ satisfied grin on their faces. The reason for conducting the research is to know how the colors of our favorite candy-coated sugary rewards came to be and that method of knowing is what we
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is the best method of separation used in candy chromatography. Paper chromatography is a technique that involves placing a small dot or line of sample solution onto a strip of chromatography paper.. The paper is placed in a jar containing a shallow layer of solvent and sealed. As the solvent rises through the paper‚ it meets the sample mixture‚ which starts to travel up the paper with the solvent. This paper is made of cellulose‚ a polar substance‚ and the compounds within the mixture travel farther
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Paper chromatography is one of the easiest methods of chromatography. It is a method of planar chromatography (stationary phase is in form of a plane). Paper chromatography follows the basic principle of chromatography‚ which states that substances or components are distributed in between the stationary phase and the mobile phase. It is an analytical technique‚ where only a small amount of a sample is used for separating and identifying its components. Like any other method of chromatography‚ paper
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Preparation and melting point of Aspirin Aspirin is a painkilling drug and is produced worldwide to reduce and stop pain. Aspirin is made up of ehtanoylation of 2-hydroxbenzenecarboxylic acid in a presence of phosphoric acid. AIM: The aim of the experiment is to prepare and produce aspirin as pure as possible. I will do this threw preparing the aspirin and purifying the product threw recrystallization. I will then obtain a melting point of my product to get an estimate of its purity. By the end
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Procedure The procedure stated in Chem 2120 experiment 6 Williamson Ether Synthesis of Phenacetin laboratory manual was followed without any major changes. Data and results Compound Amount used MW (g/mol) Moles Stoichiometry/Comments acetaminophen 0.354 g 151.16 2.34 x 10-3 limiting reagent ethyl iodide 0.3mL 155.97 3.75 x 10-3 1.6 equiv ’s sodium ethoxide 2.6mL 68.05 3.3 x 10-2 catalyst‚ reaction solvent crude product obtained: phenacetin 0.32g
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chromatography. Hypothesis I think that there will be a high RF value in paper chromatography‚ if we use water solvent because a solvent that has a strong interaction with a chemical will easily over come attraction from the chemical for the absorbent layer and move that chemical in a given period of time. Materials Food Coloring (solute) Ethanol‚ Acetone‚ Tap Water‚ Distilled Water(solvents) Pencil Ruler Scissors Filter Paper Petri Dish Variables Independent: Type of chromatography solvent
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that‚ place the ground materials in the test tube and add 5 ml of acetone. 3. Shake for 10 seconds‚ and after 10 minutes add 5 ml of water. Then‚ add 3 ml of petroleum ether and shake for 5 seconds. The pigment will eventually be found on the upper layer; remove it with a pipette and place it in a small test tube in the brown bottle. Note: dispose of the remaining materials. 4. Draw a faint pencil line on a filter paper‚ 1.5 cm from one edge. Then‚ using a capillary tube‚ make 6 or 7 dots on the pencil
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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