of pH on amylase activity This practical allows you to: * discover how pH affects the rate of an enzyme controlled reaction * evaluate the experimental procedure Procedure SAFETY: Follow your teacher’s instructions for handling the solutions. Wear eye protection when handling the iodine solution. Investigation * Place single drops of iodine solution in rows on the tile. * Label a test tube with the pH to be tested. * Use the syringe to place 2 cm3 of amylase into the
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The first step to this experiment is being completely protected from any possible harm that could possibly occur as a consequence of the practical test. Therefore‚ a lab coat‚ safety gloves with clean hands and goggles must be worn before starting the experiment. The initial room temperature should be calculated using a thermometer and a marker is used to name beakers 1 and 2. Divide the 10 test tubes into two groups and name them 1-5 in both groups. 5 different stopwatches are used to record the
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found in and out of cells and lower the activation energy of a reaction. Amylase is the enzyme which catalyses starch hydrolysis. Alpha amylase and beta amylase are two types of amylase enzymes. The amylase which is the most commonly found in the human body is the alpha amylase. Beta amylase is mainly found in bacteria‚ fungi and plants. Amylase breakdown starch into maltose. During the process of hydrolysis‚ Amylase degrades starch by splitting the long glucose units into smaller intermediates and finally
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enzyme called amylase. Amylase is the enzyme found in your saliva. The purpose of this enzyme is to help in the digestion process (McD‚ 2002). Enzymes are biological catalyst that help speed up the reaction by lowering the activation energy. These enzymes allow your food to be digested at a faster rate (National Institute of Asian Medicine‚ 2007). There are many factors that can affect the efficiency of enzymes which include pH levels‚ the amount of substrate‚ inhibitors‚ and temperature (Your Mom‚
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Conclusion 1: In this first lab we attempted to discover which of the materials given to us were hydrates by heating them and then dissolving them in water. Our results showed that all of our substances were hydrates besides the sucrose‚ sucrose if the only one which did not both bring condensation when heated and dissolve in water‚ which agreed with our hypothesis because we also thought that sucrose was going to be the only one that wasn’t a hydrate. The only error that we may have encountered
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Conclusion In this experiment‚ we measured the mass of 4 gasses; oxygen‚ carbon dioxide‚ helium‚ and lab gas. We took a Ziplock bag‚ and turned into a non-stretchy balloon‚ and filled with each gas and measured the apparent mass then calculated the actual mass‚ then find a ratio between the mass of oxygen and the other gasses. We tried to keep the pressure about the same each time so our calculations would be more accurate. We found out that the heaviest was carbon dioxide‚ and the lightest
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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an environment that is to basic or acidic the reaction will take longer to digest the starch because the enzyme shape has changed and the substrate will have a harder time connecting to the active
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Lab report Aim : To check the presence of starch in the leaves Hypothesis : the presence of starch can be detected if the specimen turns black blue after iodine solution. Materials : 1) Green leaves 2) Wire gauze 3) Iodine solution 4) Forceps 5) Specula 6) Bunsen’s burner 7) Test tube 8) Water 9) Tile 10) tripod stand 11) glass 12) slide 13) dropper 14) water 15) water bath Procedure: collect dark green leaves (as leaves contain starch) take a water bath and let it heat
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In conclusion‚ after conducting the lab‚ the emergent properties of water were tested and proved. These trials and experiments were able to verify the adhesion‚ cohesion‚ polarity‚ temperature stabilization‚ solvency‚ and density changes exhibited by water. During the glass and wax paper lab‚ we observed the adhesive properties of water. When a droplet of water touched the glass‚ it dispersed immediately. The droplet placed on the wax paper remained intact and in droplet form. Also‚ when the
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