the change to reestablish an equilibrium. The initial conditions prior to any change can be viewed as a state of equilibrium because no reaction was occurring and therefore no products were being formed. However‚ once peroxidase was added to hydrogen peroxide‚ oxygen gas rapidly started to form and began to quickly react with guaiacol to produce tetraguaiacol in an attempt to reconstruct equilibrium. Because with every minute passing there was a larger amount of the product than the minute before
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backgrounds. Activity A’s background is to investigate the specific reaction of the decomposition of hydrogen peroxide by the enzyme‚ catalase. Hydrogen Peroxide decomposes slowly into water and oxygen‚ and the addition of catalase lowers the activation energy of the reaction until it proceeds to a room temperature. The catalase will is unchanged and is usable for to catalyze the reaction of more hydrogen peroxide. The background of activities A‚ B‚ and C is to determine the concentration of H2O2
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| |Liver did not change in size or shape |peroxide was added) | | | |Bubbles rose and was large in size |2 | | | |(after hydrogen peroxide was added) | | |PART B |Liver in
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activity of an enzyme to the point where is no longer serves its vital function. Three factors are temperature‚ pH and substrate concentration. Catalase is an enzyme that is found in most living cells in the blood that catalyses the decomposition of hydrogen peroxide into water and oxygen. Aim: to test the effect of temperature increase‚ change in pH and change in substrate concentrations on the enzyme; catalase‚ in the form of potato Hypothesis: the optimum temperature for catalase is room
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I. Aim The aim of the three experiments is actually to investigate the effects of different factors such as temperature‚ pH‚ and concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase
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2MnO4- + 16H+ + 5C2O42- ⎯→ 10CO2 + 8H2O + 2Mn2+ Notes: 1. Mn2+ is colourless 2. In the reaction‚ the purple colour of the solution turns into colourless. MnO4-: Manganate(VII) ion (Purple) Mn2+: Manganese(II) ion colourless Decomposition of Hydrogen Peroxide 2H2O2 - ⎯→ 2H2O + O2 Catalyst: manganese(IV) oxide(MnO2)‚ Iron(III) oxide (Fe2O3)‚ silver (Ag) Haber Process (Manufacture Ammonia) N2 + 3H2 ⎯→ 2NH3 (More detail in Form 4 Chapter 9) Contact Process(Manufacture Sulphuric Acid)
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is to see how pH affects the activity of potato tissue catalase‚ during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase + 2H2O2 Catalase + 2H2O +O2 Catalase + Hydrogen Peroxide Catalase + Water + Oxygen Independent Variable The independent variable in this investigation is pH. Each individual enzyme has it’s own pH characteristic. This is because the hydrogen and ionic bonds between –NH2 and –COOH groups of the polypeptides that make
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the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which slowly decreases in the course of an hour. This decrease in
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presence of ethanol on the rate equation. It is known that ethanol effects hydrogen peroxide and therefore it has an effect on the rate equation. This aim will find out the effect of ethanol by carrying out the iodine clock reaction with and without ethanol present and the results will be compared to draw a conclusion. A2 AIMS: 4. Investigate the order of reaction with respect to hydrogen peroxide‚ iodide and acid. The goal of this aim is to find out what order of reaction
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hypothesis: If the conversion of hydrogen peroxide to water and oxygen by the enzyme catalase is observed ‚ the amount of oxygen generated can be measured and the rate of the enzyme catalyzed reaction can be calculated. The primary reaction catalyzed by catalase is the decomposition of H2O2 to form water and oxygen. ------------------------------------------------- 2H2O2 → 2H2O+O2(gas) ------------------------------------------------- Catalase + Hydrogen Peroxide --> Complex --> Catalase
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