milk = 87.1% water‚ 4.9 % CHO 3.9 %‚ fats‚ 0.7% minerals Experiment 2: Protein Hydrolysis and Characterization | Reagents | Principle | Test for | Positive Result | Negative Result | Biuret | CuSO4‚ NaOH | Complexation of Cu+2 with amide N atoms | Polypeptide bonds | Violet/purple solution | Blue color solution | Sakaguchi | 10% NaOH‚ 0.02% α-naphtol solution‚ 2% NaOBr | arginine condenses with α-naphtol and NaOH | Guanido group (arginine) | orange solution | light yellow solution |
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Introduction Our body cells have natural ability to resist excessive pH changes. Metabolic activity produces acid and to a lesser extent‚ base and they hydrogen ions associated can alter the overall charge‚ configuration and function of various proteins. The majority of acid results from carbohydrate and fat metabolism‚ producing CO2‚ which combines with H2O to form H2CO3‚ carbonic acid‚ dissociating to form H+ and HCO3- ions. Most bases come from metabolism of anionic amino acids and oxidation
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Denaturation is carried on by heating the double-stranded DNA at 94°C to separate the complementary strands that will serve as template in further cyclings. Pre-denaturation is sometimes done at the same temperature to ensure complete separation of strands. Annealing then occurs upon rapid cooling of the solution‚ allowing oligonucleotide
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will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism: 2 H2O2 ------------------>____2 H2O+ O2 Materials: • 1M HCl • 6 test tubes • hot water bath • graduated cylinder • 1M • • NaOH • test tube rack • ice bath • 100 ml beaker • H2O2 • test tube holder Safety: HCl and NaOH are strong acids and bases. If you spill these chemicals on your skin‚ immediately wash in running water. Wear goggles at all times. Be careful
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ABSTRACTDuring my experiment‚ I have been investigating my research question: To what extent do white and brown eggshells differ in respect to percentage by mass of calcium carbonate content?Calcium carbonate is a substance found in the eggshells giving them hardness and strength. It is essential to the commercial egg industry that the biggest possible amount of eggs reaches the market. Hence‚ as calcium carbonate reacts steadily with hydrochloric acid to produce carbon dioxide as well as two other
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first prepared from a hog pancreas and was completely neutralized using 0.5% Na2CO3. Afterwards‚ 3 mL each of the neutral pancreatin solution was placed in 7 test tubes and were further added with other specified reagents. The test tubes containing HCl were not effective in digesting the biomolecules since pancreatin is only active in moderately alkaline conditions‚ as observed in test tube 2. Data obtained from test tubes 3-7 were inconclusive and would most likely subjected to errors‚ thus‚ producing
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the ionization of a weak organic acid such as acetic acid by NaOH. CH3COOH(aq) + NaOH - + CH3COO Na + H2O As more of the strong base (titrant) is added to the aqueous solution‚ more of the weak acid is converted to its conjugate base. During this process‚ a buffer system forms and the pH of the system will follow the HendersonHasselbalch relationship. The titration curve of the neutralization of acetic acid by NaOH will look like this: pH 0.5 equival ent s pH = pKa
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Experiment #1: Carbohydrate Digestion • Tube 1 Digestion Lab – 3 ml water • Tube 2 – 3 ml 0.2% amylase • Tube 3 – 3 ml 0.2% amylase + 10 drops of 1.0M HCl • Tube 4 1 2 4 3 – 3 ml 0.2% amylase – place in hot water bath for 5 min Experiment #1: Carbohydrate Digestion • Add 5.0 ml starch solution to each tube • Incubate in 37°C bath for 1.5 hr • Divide contents of each tube evenly into 2 tubes – Lugol’s Test – Benedict’s Test Experiment #1: Carbohydrate
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heat denaturation should be added. Significance: An improved method of Taq isolation offers a valuable resource for PCR. SUMMARY The aim of this experiment was to isolate recombinant Taq polymerase from E. coli bacteria induced by IPTG. This was done successfully through differential centrifugation‚ ammonium sulfate precipitation‚ and heat denaturation as confirmed by Western Blotting‚ rt-PCR and PCR reactions. The isolated protein sample was found to still contain remnants of host proteins as confirmed
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the buffer (Tris-HCl in this case) as the mobile phase and an electrical source/ cathode and anode terminal. The aim of SDS-PAGE is to separate peptide chains in a protein sample according to molecular weight by sieving them through a gel matrix under the influence of an electric current. The setup for an SDS-PAGE experiment is shown in Figure 1.0 [pic] Figure 1.0- An illustration of a simple SDS-PAGE setup Based on the figure above it can be observed that big proteins (high MW) travel a
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