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    that the moles of the acid in the solution are equal to the moles of the base (Survey of Chemistry lab manual). Using a dilution equation of M1xV1=M2xV2‚ the concentration of the hydrochloric acid can be calculated. M1=NaOH(molarity)‚ V1=NaOH(volume)‚ M2=HCl(molarity)‚ V2=HCl(volume). Procedure: Instructions for laboratory were found on page 91 of Survey of Chemistry lab manual. All calculations were rounded off by 2 decimal places for accuracy. Preparation of the indicator required a combination

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    DISCUSSION In this experiment‚ we will extract plasmid DNA that carrying pKan and pAmp from E. coli that have been cultured overnight in LB media containing antibiotic kanamycin and ampicillin respectively. So‚ the DNA that will be introduced later will make the bacteria resistance to antibiotic kanamycin or ampicillin. A plasmid is a small‚ circular‚ double stranded DNA molecules and cloning vector that are widely used for recombinant DNA technology. It can be physically separated from chromosomal

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    HNO3 and H2SO4 have the same molarity. Why did H2SO4 require about twice the volume of NaOH solution for the titration? 2. Explain the effect of each of the following sources of error on the molarity of NaOH as determined in this experiment stating whether the concentration of NaOH obtained would be too high or too low. a. The buret is contaminated with an acid solution. b. The buret contains a large air bubble in the tip‚ which

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    prelab 8

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    Place the penny in a 50 mL of a pre-determined concentration of HCl solution overnight. 3) Then determine the percent copper from the mass of copper. 4) Find the percent of zinc by precipitation and titration 5) If there is a known amount of HCl added to the beaker that penny is placed in‚ then determine the amount of HCl that was used up in the reaction of HCl and Zinc. Calculate the % of zinc in penny. 6) If zinc reacts with NaOH in the titration experiment‚ then zinc hydroxide will form. Check

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    Worked solutions to student book questions Chapter 4 Analysing acids and bases Q1. Antacid tablets should normally be chewed before they are swallowed. Why? A1. Antacid tablets are normally chewed to provide a larger surface area for faster reaction with stomach acids. Q2. A laboratory test to determine how much hydrochloric acid is neutralised by a brand of antacid does not give a complete picture of its effectiveness in the stomach. What other factors might be important? A2. Other factors to

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    |[pic] | | | | |UNIVERSITI TEKNOLOGI MARA | | | |FAKULTI KEJURUTERAAN KIMA | | | |CHEMICAL ENGINEERING LABORATORY III | | | |(CHE575)

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    unknown solution used‚ the unknown concentration can be calculated. For example‚ standard base solution (NaOH) is added from a burette to an accurately known volume of the acid solution (HCl). HCl(aq) + NaOH(aq) ------------> H 2 O(l) + NaCl(aq) This reaction (neutralization) can be written as a NET IONIC equation as follows: H+(aq) + OH-(aq) ----------> H 2 O(l) (2) (1) When sufficient NaOH has been added to react with all of the acid‚ the titration is complete -- the equivalence point has been

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    experiment acid-base titration will be used to determine the concentration of HCl at equilibrium when it reacts with the NaOH H+ (aq)+ Cl- (aq) + Na+ (aq) + OH- →H2O(l) + Na+ (aq)+ Cl- (aq) Procedure When performing this experiment one must first obtain and wear goggles. Next add 40mL of distilled water to a 100mL beaker‚ then add 5.00mL of HCl to the beaker.Then obtain 40mL of 0.1M NaOH. Place the NaOH in a 60mL reagent reservoir and drain a small amount into a 250mL beaker to fill

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    Indicator 1. Fill the 50-milliliter buret with a 0.25 molar NaOH solution. 2. Record volume. 3. Measure out between 20 milliliters and 40 milliliters of the unknown HCl solution. 4. Record volume. 5. The amount of unknown HCl is then added to the 100-milliliter Erlenmeyer flask. 6. Add two drops of the indicator‚ phenolphthalein‚ to the acid in the flask. 7. Using the slider on the right hand side‚ add NaOH to the HCl in the Erlenmeyer flask (This action is known as titrate)

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    Titration Lab

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    Lab Report: Titration Lab Prepare a solution of a given concentration; understand titration including acid-base reactions‚ pH‚ stoichiometry and molar equivalence. Chemicals and equipment: NaOH pellets close to purity‚ HCl 3M‚ phenolphtalein Beckers‚ flasks‚ burette‚ magnetic or manual stir pHmeter Waste management: The waste disposal will be handled through neutralization of your excess reactant to a pH between 4.0 and 10.0 and disposal with abundant rinsing. As a preparation for the

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