acid was determined by establishing a titration curve with the pKa values and pI point and molecular weight were either directly or indirectly found through the titration curve. INTRODUCTION Amino acids are also known as the building blocks of proteins. They are constituted of the amine group –NH3‚ the carboxyl group –COOH‚ hydrogen and “R” group which is different for each amino acid. The “R” group gives the amino acid its name. in physiological systems where the pH is near neutrality‚ the amino
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[PARVATIBAI CHOWGULE COLLEGE(H.S.S)-MARGAO-GOA] Chemistry chart Std-XII Name______________________________________________ Class____________________________________ Div__________________ Roll No___________ Batch No_______ 1 [PARVATIBAI CHOWGULE COLLEGE(H.S.S)-MARGAO-GOA] Chemistry chart Std-XII VOLUMETRIC ANALYSIS REDOX TITRATIONS Experiment No:- 1 Date:--------------- Aim:- You are provided with two solutions as follows. Container A: -----------------M/N Stock Solution of Hydrated
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enzymes are protein with the ability to enhance the rate of reaction between molecules. To catalyze a reaction‚ the enzymes have to create the best environment that is called active site. The active site has to be fitted with the reactant or substrate for the enzyme’s reaction. Therefore‚ most enzymes are very selective‚ and specific. Enzymes can show the best efficiency
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Phosphatase | BCHM 405 | Aaron BANESEH 10307275 | Table of Contents 1 Table of Contents 2 2 Introduction 4 3 Experimental Procedures 5 3.1 Sample preparation 5 3.2 Initial Extraction of Acid Phosphatase 5 3.3 Determination of Protein Concentration by the Folin-Lowry Assay 5 3.4 Specific Activity Assay 6 3.4.1 Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of Michaelis-Menten Constant 7 3.7 Ammonium Sulphate
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laboratory‚ the data collected from the experiment will be used to graph the titration curves which help to identify an unknown amino acid. IntroductionAmino acids are simple monomers which are strung together to form proteins. Amino acids play a key cellular role in structure and function. Proteins themselves participate in nearly every physiological event in the cell. Since all amino acids contain at least one amino and one carboxyl group‚ they are classified as amphoteric substances (meaning that they
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separation‚ and (3) chemical denaturation. In the first step‚ the DNA sample is digested with three restriction enzymes that were picked as part of a triple digest plan. The result of which can be used to make a restriction map of the DNA of interest. Secondly‚ after the digestion of the sample‚ the agarose gel can now be run to separate the fragments by size. In step three‚ which is performed twice‚ the gel from the previous step it soaked in a bath of sodium hydroxide (NaOH) and placed on a shaker.
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are electron acceptors 3.Ferichydroxide is insoluble precipitate and has ………………….. a) blue color b) white color c) brown color d) dirty green color 4.The reduction of alkylhallides takes place in the presence of a) Zn/HCl b) Na/HCl c) Mg/HCl d) Cu/HCl 5.Deficiency of vitamin E causes a) rickets b) scurvy c) anemia d) night blindness 6.Which one of the following is reducing sugar? a) glucose b) maltose c) sucrose d) starch 7.Buildings are being damaged by
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acid‚ CH3COOH. • • • • • a strong acid: hydrochloric acid‚ HCl. an acidic commercial cleanser. a basic commercial cleanser. to use the titration curves to calculate the percent of the active ingredients in the commercial cleansers. to determine the Ka of a weak acid. Procedure Overview • • • • after calibration of the pH electrode and determination of the flow rate for the automatic titration‚ a NaOH solution is standardized against HCl. a pH titration curve for acetic acid is obtained and its pKa
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scale Procedure: Step 1: Standardization of the NaOH solution using Standard 0.1M PHP 1. Take 10ml of the given NaOH solution and dilute with 90ml of water. Fill up the burette with this solution. Take 10ml of 0.1M potassium hydrogen phthalate (PHP) in the conical flask‚ add 1-2 drops of phenolphthalein and titrate with the diluted solution of the NaOH till the solution turns light pink. 2. Calculate the strength of the original NaOH solution. Step 2: Poultry eggshell 1. Carefully
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of Na2CO3 through the preparation of NaOH and HCl standards. The molarity of the standards will be found through titration of KHP for NaOH‚ HCl vs the known NaOH‚ and the unknown Soda Ash sample vs the known HCl. II. Equations and Sample Calculations: Titration of HCl with NaOH: Complete Equation: HCl (aq) + NaOH (aq) H2O (l) + NaCl (aq) Net Ionic Equation: H+ + OH- H2O(l) Calculations (10mL 0.1M HCL‚ 100mL H20‚ 7.8mL NaOH): # mols NaOH = 0.1M (known concentration) x 0.0078L
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