Molarities Through Dialysis Tubing (a Semi-Permeable Membrane) I. DESIGN A. PROBLEM/RESEARCH QUESTION 1. How does increasing molarity of sucrose affect osmosis through dialysis tubing? B. VARIABLES 1. The independent variable in this lab is the molarity of sucrose each dialysis bag is filled with. The time (30 minutes)‚ the temperature (23C) and the type of dialysis tubing used are all constants. 2. The dependent variable is the final mass of the dialysis bag.
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Introduction: In this lab i used glucose and starch to show if the cell membrane is permeable‚ impermeable‚ or selectively permeable to these substances. Dialysis tubing is a plastic like substance that has small pores in it. These pores allow certain chemicals to flow into and out of the cells. The size of the molecule determines what goes through the tubing. On of the materials that we will be using is glucose. glucose is a simple sugar that easily dissolves in water. To test for the presence of glucose
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solution that has less solute is hypotonic. 3. This experiment was performed to measure the osmosis of small molecules through cellulose bag (has the same structure that plant cell does). This tubing acts as a selectively permeable membrane‚ allowing larger molecules to pass through‚ but slowly. Dialysis is the movement of a solute through a selectively permeable membrane. Purpose: To observe osmosis in a model membrane system. Hypothesis: If I immerse in the beaker containing water one bag
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osmosis in the cell. In this case‚ we used dialysis tubing to simulate the semi-permeable membrane of an animal cell. Materials and Methods: Dialysis tubing (6 x 30cm) String for tying dialysis tubing Clear plastic drinking cups Sharpie Markers Funnels Glucose/starch solution Distilled water IKI solution Glucose TesTape 0‚ .2‚ .4‚ .6‚ .8‚ and 1 Molar Sucrose Solution Cork Borer Potatoes Scale Exercise 1: Diffusion Fill a dialysis bag with a sugar/starch solution and immerse
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The effect of size on the effectiveness of diffusion Aim: To use agar blocks infused with 0.1 Molar sodium hydroxide (NaOH) and phenolphthalein to investigate the relationship between shape and surface area: volume ratio on the effectiveness of diffusion. Hypothesis: That for a cube of agar‚ the time taken for complete colourisation due to diffusion of HCl is directly proportional to the cubes volume. Materials: |A block of agar (10cm x 5cm x 3cm) with 0.1M NaOH and |1x 250mL
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Procedure Diffusion Jaydon‚ Jacob and Tyler obtained a 30 cm piece of dialysis tubing and cut 2.5cm off of the 30cm piece and soaked it in water. Then they tied one side off to form a bag. They then rubbed the other end of the bag to open it up and then are able to fill the bag with solution. The next step of the lab was to test for the presence of glucose in a 15% glucose and 1% starch. Then they took the 15% glucose and 1% starch and took 15 mL and placed them in enclosed bags. Jaydon‚ Jacob
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Part I: Pre-Lab Diffusion/Osmosis Pre-Lab Introduction: Dialysis tubing allows molecules to diffuse through microscopic pores in the tubing. Molecules that are smaller than the pores can diffuse through the dialysis membrane along the concentration gradients. Molecules that are larger than the pore size are prevented from crossing the dialysis membrane. Answer questions in complete sentences. For problems‚ show equations and work with units and appropriate significant figures. Part IA: In the following
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add higher concentrations of sugar to the dialysis tubing‚ then the net movement of coffee into the dialysis tubing will increase. Materials scale or balance 24" dialysis tubing 4 transfer pipets sugar scissors rubber bands four coffee cups - they need to be roughly the same size 250ml graduated cylinder ruler small sauce pan 3 clean containers (600mls (about 20 ozs) or larger) Procedure: 1. Cut four 6-inch pieces of dialysis tubing and soak in a coffee cup filled with tap water
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diffusion gradient forming a hypertonic or hypotonic solution. Solvent with equal or no solute forms an isotonic solution. Materials: Distilled water‚ sucrose‚ dialysis tubing‚ string‚ 250 ml beaker. Procedure: To demonstrate and isotonic solution we needed 3 inches of dialysis tubing. We tied off one end of the dialysis tubing to create a bag‚ filled it with distilled water‚ and tie of other end to close. The bag is carefully blotted to remove any water spilled during the filling and then
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After searching for a while and scratching out ideas we found a two inch PVC pipe along with two one and a half inch test plugs that are water tight. At this point we still had the water idea to hold the egg inside the tubing. To keep the wieght down we used cotton balls inside the tubing to keep the egg from coming in direct contact with the hard plastic‚ but instead with the soft cotton. With this idea secured we took ot to the testing stage. After wrapping the egg in cotton and securing it in the
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