Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
Premium Catalase Enzyme Chemical reaction
Lab 4 Quiz 1. Enzymes and what is their function? a. Enzyme: biological catalysts that regulate chemical reactions that occur in cells. Responsible for things such as converting food to energy‚ replace old damaged tissues‚ disposal of cellular waste products. Are responsible for lowering the activation energy. 2. Most enzymes are proteins with three-dimensional shapes determined by their amino acid sequence. 3. Substrate – is a reactant molecule that binds to the highly specific active site
Premium Enzyme
_____ 3. Questions 15 points/ _____ Lactase enzyme lab OBJECTIVES: 1. Understand how biological molecules are essential to the survival of living organisms * * 2. Compare the structures and functions of the major biological molecules(carbohydrates‚ proteins‚ lipids‚ and nucleic acids) as related to the survival of organisms. * 3. Investigate and describe the structure and function of enzymes and explain their importance in biological systems
Premium Glucose Enzyme Fructose
Abstract: After reviewing the basics of enzymes and catalysis‚ we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet
Premium Enzyme Protein Catalysis
Experiment Report Title: Water Conductivity Level in Goa Gajah Research Question: How high is the water conductivity level of the water sources in Goa Gajah? Hypothesis: I predict the level of electrical conductivity of the water sources in Goa Gajah will be between the range of a normal drinking water‚ which is 0.0005 to 0.005 s/m. This hypothesis is based on the fact that conductivity largely concerns itself with the amount of salt in the water‚ and since none of the water sources are
Premium Water Body of water Electrical conductivity
Water is a chemical compound with the chemical formula H2O. A water molecule contains one oxygen and two hydrogen atoms connected by covalent bonds. Water is a liquid at standard ambient temperature and pressure‚ but it often co-exists on Earth with its solid state‚ ice‚ and gaseous state (water vapor or steam). Water also exists in a liquid crystal state near hydrophilic surfaces. Water is considered a purifier in most religions. Major faiths that incorporate ritual washing (ablution) include Christianity
Premium Oxygen Metabolism Atom
Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
Premium Catalase Bacteria Hydrogen peroxide
Figures Abstract An experiment was designed and conducted to confirm Archimedes’ theory which states that an object will float in water providing its mass is equal to or less than the volume of water it displaces. The second objective of the experiment was to produce a value for water density. It was expected that when additional mass was added to a floating vessel‚ it would displace more fluid and the increasing submerged volume of water could be measured‚ and a value for water density could be calculated
Premium Density Measurement Water
Exercise 11: Blood Analysis: Activity 1: Hematocrit Determination Lab Report Pre-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1. Hematocrit Your answer : a. refers to the percentage of white blood cells (WBCs) in a sample of whole blood. Correct answer: b. of 40 means that 40% of the volume of blood consists of RBCs. 2. A buffy coat layer You correctly answered: d. is all of the above. 3. The diagnosis of anemia indicates You correctly answered: a. a lower-than-normal
Premium Red blood cell Hemoglobin Blood
The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
Premium