Investigating the Effect of Concentration on the Rate of Enzyme Catalysed Reaction To investigate the effect of concentration on the rate of enzyme catalysed reaction I must know all the factors that affect it in order to investigate in this. Here are all the factors: Temperature- Enzyme like it warm but not too hot. Enzymes are biological catalysts‚ which speed up chemical reactions. They work most efficiently at the optimum point (37°C). If they are below 37°C they will work
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Investigating the Effect of Enzyme Concentration on Pectinase in the Production of Fruit Juice In this practical‚ I shall demonstrate the relationship between the concentration of pectinase and the amount of fruit juice produced accordingly in proportion. Apparatus: Apples Pectinase solution 10 x filter paper Knife or kitchen mincer Glass rod Water bath X 1 250 ml beakers X 2 Wash bottle of distilled water 100 cm^3 measuring cylinders In the case
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Question Give an account of how enzymes work with particular reference to factors that affect their activities. Answer All living things require catalysts to catalyse the reaction which they carry out to survive. Enzymes are the catalysts of biological systems without enzymes reactions in cells would occur all much to slow a rate and the organism would not survive. Most enzymes‚ with the exception of some RNA catalysts‚ are proteins. They often require a co factor such as zinc or iron‚ or a complete organic
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Enzyme concentrations Hypothesis The higher the concentration of protease enzyme the higher the rate of breakdown of the egg white. This is because egg white is mainly formed of collagen‚ which is a protein‚ which is what the enzyme protease breaks down. The more enzyme there is the more enzyme substrate complexes are formed which break down peptide links between the monomers of the protein accelerating the amount of protein broken down in a space of time. How the results will be obtained
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Catalytic Groups in Enzyme Active Sites Thomas K. Harris1 and George J. Turner2 Department of Biochemistry and Molecular Biology‚ University of Miami School of Medicine‚ Miami‚ Florida 2 Department of Physiology and Biophysics and the Neurosciences Program‚ University of Miami School of Medicine‚ Miami‚ Florida 1 Summary In protein and RNA macromolecules‚ only a limited number of different side-chain chemical groups are available to function as catalysts. The myriad of enzyme-catalyzed reactions
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Hydrolysis‚ Solubilization and Enzyme Inactivation D. Krlrç Apar and B. Özbek* Yrldrz Technical University‚ Department of Chemical Engineering‚ Davutpaºa Campus‚ 34210‚ Esenler/Istanbul‚ Turkey Original scientific paper Received: April 11‚ 2007 Accepted: May 31‚ 2007 The aim of this study was to investigate the influences of substrate concentration‚ enzyme concentration‚ temperature and pH on hydrolysis and solubilization of corn gluten as well as enzyme stability. The corn gluten was
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Abstract Enzymes are proteins that lower the activation energy needed for chemical reactions. The two main environmental factors that can affect the enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature
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INTRODUCTION Enzymes are biological catalysts that speed up chemical reactions‚ without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a ’ball’ shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme‚ a product is produced. Enzymes are specific to their
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Chapters 6‚7 &31 Question 1 1 out of 1 points Some enzymes contain molecules in the active site that help facilitate chemical transformations. These molecules are called: Selected Answer: coenzymes. Correct Answer: coenzymes. Question 2 1 out of 1 points People with high cholesterol levels often take drugs in an attempt to lower their cholesterol levels. One such drug is Lipitor. How does this drug work? Selected Answer: It acts as a competitive
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Starch can be hydrolyzed into simpler carbohydrates by acids‚ various enzymes‚ or a combination of the two. The resulting fragments are known as dextrins. The extent of conversion is typically quantified by dextrose equivalent (DE)‚ which is roughly the fraction of the glycosidic bonds in starch that have been broken. These starch sugars are by far the most common starch based food ingredient and are used as sweetener in many drinks and foods. They include: Maltodextrin‚ a lightly hydrolyzed (DE
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