Enzymes Ashley Njeru Mrs. Booth Tuesday‚ March 19th 2013 SBI 4U INTRODUCTION: The cellular activity of all organisms is controlled through the use of enzymes. An enzyme is a special type of biological molecule‚ usually a protein‚ which speeds up a chemical reaction; most are soluble in water or a dilute salt solution. There are about 4000 different enzymes in a typical living cell. If even one of these enzymes is missing or
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Pablo Cuate Biochemistry Lab Report Objective: The purpose of this lab is to analyze enzyme activity at pH neutral for acid phosphatase as the colorless p-nitrophenylphosphate (PNPP) substrate concentration changes. We will empirically determine the concentration of production formation‚ Vmax and Km from absorbance of yellow PNP in the reaction. Results Figure 1 This figure indicates a positive linear trend for the concentration of PNP and absorbance. Table 1: Absorbance Value for Acid Phosphatase
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The kinetics of the Harcourt-Essen Reaction (Hydrogen peroxide variation) Aims: To find the order of the reaction with respect to the Hydrogen Peroxide (H2O2)‚ Potassium Iodide (KI) and the Sulphuric Acid (H2SO4) by the use of an Iodine clock reaction. Calculate the rate constant‚ mechanism and equation Find the effects of temperature on the rate of reaction The effects of a catalyst on the rate of reaction Find the activation enthalpy (Ea) of the reaction‚ with and without a catalyst Background:
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Kinetic study of a Solvolysis (Sn1) Reaction Aneadra Bowles Adasia Rutledge Krystal Flakes Robert Grimes Jasmine Ross June 5‚ 2012 Purpose: The purpose of this experiment is to use kinetics to study a solvolyis reaction Introduction: The purpose of this experiment is to understand the kinetics of the hydrolysis of t-butyl chloride.The kinetic order of reaction was studied under the effects of variations in temperature‚ solvent polarity‚ and structure. It is particularly observed in
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AP Chemistry Kinetics Lab Abstract: The purpose of this lab is to learn how to write law expressions‚ determine orders by graphs‚ and calculate rate constants. By determining the number of drops of sodium hypochlorite necessary to make a diluted food dye change to colorless in less than three minutes helps calculate the absorbance which can then be analyzed to find the pseudo rate constant and eventually leading to the rate constant and the rate law. The results show that the m and n are both 1st
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Enzymes An enzyme is a protein used to speed up the rate of a chemical reaction. Because they regulate the rate of chemical reactions‚ they are also called catalysts. There are many‚ many different types of enzymes‚ because for each chemical reaction that occurs‚ an enzyme specific to that reaction must be made. To act on a substrate‚ an enzyme must contain an active site. The active site is the area on the enzyme that allows the substrate and enzyme to fit together. The amino acids that are present
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Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes‚ and to investigate the effect of temperature‚ surface area‚ pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1
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Enzyme action is the simple mechanism by which enzymes catalyse chemical reactions. This begins with the binding of the substrate to the active site on the enzyme. The binding of the substrate to the enzyme causes changes in the distribution of electrons in the chemical bonds of the substrate. This then causes the reactions that lead to the formation of products that are then released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique shape that
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Change‚ Maxima & Minima Problems‚ Derivatives of Trigo Function‚ Exponential & Log Functions‚ Integration‚ Area Of a region‚ Kinematics‚ Curves & Circles‚ Plane Geometry. 3. Ten Years Series‚ Redspot Book Chemistry 1. Secondary Three Topics- Kinetic Particle Theory‚ Measurement & Experimental Techniques‚ Purification & Separation‚ Elements Compounds & Mixtures‚ Atomic Structure‚ Ionic Bonding‚ Covalent & Metallic Bonding‚ Writing Eqn‚ The Mole‚ Chemical Calculations‚ Acid & Bases 2. Secondary
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Enzyme are a very important component in living organisms. If enzymes did not exist than life itself would not exist (Lab Manual 3 pg. 1). They help in many different ways that are useful to the body of living organisms. Enzyme are used to speed up chemical reactions (Lab Manual 3 pg. 1). Through this process‚ they are considered very unique because they are not altered or consumed within the reaction (Lab Manual 3 pg. 1). This is why enzymes are considered biological catalysts. They also do not
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