MBK – Lab Report Name: ____________________ Section: ___________________ Module 3‚ Experiment 3: Aseptic Technique & Culturing Microbes Part 3: Generating Microbial Cultures: Observe your culture tubes after 24 hours to assess the growth patterns of all tubes. If there is no observable growth allow the tubes to incubate an additional 24 hours. Record your observations here. Attach a picture of you incubator in this space. Questions: A. What is
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Aseptic Technique and Culturing Microbes‚ Lab #3 Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media‚ including MRS broth and nutrient broth‚ learn about oxygen and temperature requirements for microbial growth and how to control microbial growth. Procedure:
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Some organisms were too large to measure. Antibiotics were susceptible to bacteria and had less growth‚ whereas antiseptics were resistant to bacteria‚ and had more growth. Antiseptic of S. epidermidis had more growth than antibiotic of S. epidermidis. Antiseptics of S. epidermidis were most effective and Mr. clean had more growth all over the agar. S. epidermis of antibiotic were the most effective because it was susceptible and Chloramphenicol and tetracycline had a little bit of
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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CHAPTER 1 INTRODUCTION One of the most common infections that we can easily get was skin infections caused by bacteria‚ specifically Staphylococcus aureus and Staphylococcus epidermidis. These two Staphylococcus species can found worldwide‚ they are a small component of soil microbial flora. We have different treatments for skin infections‚ but in those treatments only few people can afford it. Because most of them are expensive for ordinary people who are usually
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The Effect of Acetyltransferases on 2 different strains of E. coli Introduction Scientists have recently discovered that resistance to antibiotics may not be such a new thing. Evidence of bacteria samples in Canadian permafrost proposes that these resistances have been around for at least 30‚000 years (Luiggi 2011). In our required pre-lab reading‚ we learned tuberculosis is becoming increasingly drug-resistant‚ giving proof that bacteria can adapt to necessary changes in order to survive (Barry
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the primary stain is called gram-positive. Bacteria stain differently because of chemical and physical differences in the cell wall. Procedure For this lab we used three cultures; Escherichia Coli‚ Bacillus Subtilis‚ and Staphylococcus Epidermidis. Using a different slide for each sample we started by preparing and fixing each slide. Next we covered the slide with crystal violet for 30 seconds then rinsed with water. Next we covered the slide with Grams Iodine for 10 seconds and rinsed with
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knowledge and skills in making observations about specimens. Methodology: Materials: -Small cardboard box or Styrofoam Cooler -Microscope -Immersion Oil -Desk Lamp -Aluminum Foil -Paper Towels -10% bleach solution -Gloves -S. epidermidis sample -Goggles -Thermometer -Apron -Test-tube rack -Candles -Slide box with blank slides -Cover slides -Broth MRS-9ml -Broth‚ Nutrient -Lactobacillus acidophilus -Sterile swab -Gram stain solution #1 crystal violet -Mask
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line S. epidermis in liquid nutrient broth No growth Pellicle formed Procedure: After setting up the incubator‚ I aseptically transferred S. epidermidis and L. acidophilus. to generate liquid broth cultures. After waiting for 24-48 to observe growth‚ I recorded my observations. Then‚ I prepared wet mount slides and direct staining slides of both S. epidermidis and L. acidophilus. to observe them microscopically using oil immersion lens. When I was done‚ I stored them in the refrigerator for future
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