Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids‚ thus make up many different combinations in types‚ numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein‚ due to various reactions of amino acids to each
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Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
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stating that I have permission to do this test. Lab Assessment Questions and Answers 1. Provide a brief summary of the activities in each of the hacking steps listed below: 1. Reconnaissance—The initial stage of collecting information on your target network 2. Enumeration—The process of querying active systems to grab information on network shares‚ users‚ groups‚ and specific applications 3. Gaining access—The actual penetration 4. Maintaining access—Allowing the tester a backdoor
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Keith Brown IS 4670 Lab 1 Perform a Byte-Level Computer Audit 1. What is the main purpose of a software tool like WinAudit in computer forensics? Answer: WinAudit is a great free tool that will give you a comprehensive view of the components that make up your system‚ including hardware‚ software and BIOS. 2. Which items within WinAudit’s initial report would you consider to be of critical importance in a computer forensic investigation? Answer: Computer Name‚ OS‚ Security Settings for Windows
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Procedures for Part A: For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled
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Investigation Aim: The aim of the investigation was to investigate the effect of substrate concentration Hydrogen Peroxide H O (in %) on the rate of reaction of the enzyme catalase (in 1/mean time). Prediction: As the substrate concentration (hydrogen peroxide) in % increases the rate of reaction in 1/mean rate increases until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached‚ then adding extra substrate will make no difference. The
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00 Flag question Question text In a scientific experiment‚ the control group ______. Select one: a. serves as a basis of comparison with the experimental group b. is required for the validity of discovery science c. serves to increase the sample size of the experiment d. allows for the simultaneous testing of multiple variables e. is subjected to the factor whose effect is being tested Feedback Your answer is correct. Question 4 Incorrect Mark 0.00 out of 2.00 Flag question Question
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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immersion lens while observing prepared bacterial slides and also to prepare slides for observable bacteria culture from yogart. Procedure: Exercise 1: Using the Microscope - Viewing Prepared Slides I started out by reading and reviewed the science lab safety reinforcement Agreement and the instruction to oil immersion lens. After setting up my lab station I view the six prepared slides with 10x and 40x objctives lens. In each slide the differences was magnification and the 40x lans gave a clearer
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Using different sucrose concentrations Introduction The purpose of this experiment was to estimate Osmolality of plants cells i.e. Potato and celery by converting the observed change of weight in different sucrose concentrations. The hypothesis assumes that the solute concentration of the plant samples would be indirectly proportional to the weight; there would be decrease in weight as the concentration increases. The independent variable in this experiment would be the change in weight
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