Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method used for the separation of
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SOLVENT EXTRACTION Solvent extraction‚ also known as liquid extraction and partitioning‚ is a method to separate compounds based on their relative solubility in two different immiscible liquid‚ usually water and organic solvent. It is an extraction from one liquid phase to another liquid phrase. Liquid extraction is a basic technique in chemical laboratories‚ where it is performed using a separator funnel. This type of process is commonly performed after a chemical reaction as part of the work-up
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E5 & E6 Extraction of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach.
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extracting lipids or fats from foods‚ both the method as well as the solvents chosen to perform a complete‚ or close to complete extraction are important. If these two elements are not taken into consideration‚ the extraction may not be complete‚ or the extract may contain a large quantity of undesired impurities. The natural fats and oil are mixtures of glycerides of fatty acids. Fats and oils are naturally occurring organic compounds which belong to a large group of water insoluble substances called
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Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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Reacting Acids and Bases Lab Report Introduction Every liquid‚ except for distilled water‚ has either acidic or basic traits. An acid is sour tasting‚ and gives a sharp stinging pain in a cut or wound‚ and bases taste bitter‚ and feel slippery. A pH scale is used to determine what traits a liquid has; acidic or basic. The scale focuses on OH- (hydroxide ions) and H+ (hydrogen ions). The scale goes from 0 to 14. 7 is distilled water‚ as it is directly in the middle; neither acidic or basic. Acids are
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Mejia Chem 101 Section ADF Lab 4: Weak Acid Unknown Procedure: When testing the acid‚ use only between 0.2 g and 0.3 g for each trial (get as precise a measurement as you can). The general procedure is to weigh out your acid‚ dissolve it in water‚ add a couple drops of the indicator (phenolphthalein)‚ and then add the sodium hydroxide until you note a color change (from clear to pink). When the color change occurs‚ you have added enough base to completely react with the acid (the endpoint). You are
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Acetylsalicylic Acid September 15‚ 2005 September 23‚ 2005 OBJECTIVES The objective of this lab experiment is to prepare aspirin (acetylsalicylic acid) by reacting salicylic acid and acetic anhydride. INTRODUCTION This purpose of this lab was to prepare aspirin using its basic components: salicylic acid and acetic anhydride. Salicylic acid and acetic anhydride‚ along with an acid catalyst‚ react to form acetylsalicylic acid (aspirin) and acetic acid. In this reaction‚ the hydroxyl group
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Republic of the Philippines Mindanao State University General Santos City Bio 108: Cell Biology Laboratory Report August 28‚ 2013 DNA Extraction on Onion (Allium cepa) and Human Cheek Cell Arriza‚ Rolland Merch Buscato‚ Carl G. Butil‚ Conrad G. Leonida‚ Renee Theresa ABSTRACT This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed
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4/2/14 Period: 1st Chemistry Sour Acids and Bitter Bases Purpose: The purpose of this lab was to observe the different reactions formed between various acids and bases with the aid of indicators. Equipment: 1. Safety goggles. 2. Droppers. 3. Red Litmus paper. 4. Blue Litmus paper. 5. pH paper. 6. Well plate. 7. Micro spatula. Materials: 1. Zinc. 2. Magnesium. 3. Iron. 4. Copper. 5. HCL. 6. HC₂H₃O₂. 7. NaOH. 8. Phenolphthalein. Procedure: Part A: 1. Add five drops
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