Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired analyte
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Titration Lab How Much Citric Acid is in Your Soda? PURPOSE To determine the molarity (concentration) of citric acid (H3C6H5O7) in various different citrus flavored sodas. BACKGROUND PRINCIPLES Popular sodas all strive for a somewhat sour (’tart’) flavor and manufacturers utilize acids to impart this taste. While there are a vast variety of different brands of sodas on the market‚ they can be broken into two main categories: dark or clear. This differentiation is not only a simple color
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lab‚ caproic acid was synthesized in a multi-step process that involved the synthesis of three intermediates – diethyl n-butylmalonate‚ potassium n-butylmalonate‚ and n-butyl malonic acid respectively. An IR was used to characterize the starting material‚ n-bromobutane‚ and the first intermediate‚ diethyl n-butylmalonate; while IR and NMR were used to characterize the final product‚ caproic acid. Reactions‚ Mechanism and Theory Caproic acid a.k.a n-hexanoic acid is a carboxylic acid derived from
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Recrystallization of Benzoic acid Chemistry 204-002 Date: 4/9/2011 Hector Ortega Recrystallization is a method used to purify compounds‚ and this is a very important procedure because compounds are naturally impure. Polarity and solubility play a big role in this experiment and these characteristics are exploited in order to have a crystallization of the desired compound. In this experimented benzoic acid was crystallize using ethanol and water. I chose these solvents based on part C of the experiment
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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Procedure: Melting point of RCO2H Before observing the melting point of the unknown sample‚ the melting apparatus had to be standardized with a standard substance having the melting point of 151°C-153°C. After placing a small sample of the standard in a capillary tube and inserting the tube into the sample tube holder‚ the plateau on the device was set to 15°C below the expected melting point. After its temperature reached the plateau‚ the temperature increment was set at 1°C per minute‚ and the
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Experiment 4 Synthesis of Salicylic Acid Introduction Throughout history‚ botanical extracts have been used as medicines. Approximately 30% of all medicines have a plant origin. This number increases to 60% if you consider medicines that at one time were derived from plants‚ but have been synthesized in the laboratory. Salicylic acid is a white crystalline compound that can be isolated from the bark of birch trees. Since it is a valuable substance that can be isolated from nature‚ it is called
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BOILING POINTS AND MELTING POINTS PART 2 Name: Grethel Zegarra Date of Lab: October 26‚ 2010 Date lab is due: November 2‚ 2010 HYPOTHESIS The boiling point in degrees Celsius will increase as the number of carbon atoms increase. The melting point of the unknown will indicate its mixed substances. The boiling point will increase as a result of the different branching of the OH‚ the melting point will be affected by the different positioning of the OH on the benzene ring. PURPOSE To introduce
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STANDARDIATION OF ACID AND BASE Eunice Ivy B. Gamboa ABSTRACT Solutions of known concentration are prepared by dissolving measured masses of standard acids in distilled water. The concentrations of unknown solutions of sodium hydroxide are determined by titration. An acid solution reacts with a base solution in a "neutralization" reaction. Titrations permit the concentrations of unknown acids/bases to be determined with a high degree of accuracy. In order to analyze unknown acids/bases‚ we must have
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both increases as I thought they would. 2. Describe some possible causes of respiratory acidosis. Depression of the respiratory center in the medulla oblongata as well as airway obstruction may cause respiratory acidosis. 3. Explain how the renal system would compensate for respiratory acidosis. The kidneys regulate the amount of H+ ions and HCO 3- excreted in the urine which regulate acid base balance. A C T I V I T Y 3 Renal Responses to Respiratory Acidosis and Respiratory Alkalosis
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