casting tray where it remained for solidification. Once solidified‚ 300 ml of a buffer was added‚ this is so the electrical field has something to pass through. Next‚ each person pipetted 5 microliters from their PCR tubes into the wells inside the gel. Once all the samples were pipetted‚ the electrical current was added and remained running for anywhere between 10 and 20
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Discussion Questions – Urine 1. The results obtained for this urine sample (U19) shows that the patient is infected with urinary tract infection. This is identified by the high amount of colonies observed on Horse Blood Agar (>108/L)‚ mild amount of protein (30 mg/mL) and very high amount of red blood cells (Large+++) found in the urine. The bacterium identified in this specimen is Pseudomonas aeruginosa. This is confirmed by involving many tests and observation‚ such as Gram stain‚ oxidase test
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What is salmonella? According to the Mayo Clinic Salmonella infection is a common bacterial disease that affects the intestinal tract. Salmonella bacteria typically live in animal and human intestines and are shed through feces. Humans become infected most frequently through contaminated water or food sources. Salmonella serotype Typhimurium and Salmonella serotype Enteritidis are the most common in the United States. (Centers for Disease Control and Prevention) The infectious agent (pathogen)
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******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted: a. Genetic testing b. Body Identification c. Forensic analysis 3. Name the piece of equipment
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Biology 240. Spring 2014. Exam 3. Chapter 9. Proteins and Their Synthesis Four levels of protein structure (page 313) Primary: linear sequence of amino acids in polypeptide chain Secondary: local regions of polypeptide chain fold into specific shapes (shapes arise from the bonding forces between amino acids close in proximity of linear sequence Tertiary: folding of the secondary structure Quaternary: protein composed of two or more separate folded polypeptides (subunits) joined by weak
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| α-Keratin | Aliphatic (hydrophobic) | R-handed | L-handed4(2chains) | β-Keratin & Fibroin | Every other AA glycine | β-sheets | - | Collagen | Gly-X-Y Hydroxyproline/lysine | L-handed | R-handed(3chains) | Laboratory Techniques Electrophoresis - is the movement of charged
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Midterm Study Guide Answers 1. A hypothesis is an explanation of observations. “If the floor is wet‚ I will slip.” 2. A controlled experiment is when only one variable is changed. 3. If the plant you are experimenting with has a disease that is an unavoidable experimental error. 4. An enzyme speeds up reactions and lowers the energy it takes to produce something‚ a lock and key. 5. Autotrophs make their own food by producing sugars from sunlight and various chemicals. 6
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Online BIO 150 Introductory Microbiology #3 Lab Report NAME __ Lab Group 2_____ Answer the following questions as you work your way through the lab material typing in your answers. Then submit your finished lab report as a Microsoft Word document. This lab report is worth 100 points towards your final lab grade. Each Q is worth 2 points unless otherwise noted. Also‚ per the Honor Code‚ this work must be your own. This is due Mon. 10/8 at 11:59 PM. The theme of this lab is the identification
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lysozyme thanks to primer extension method‚ which was then used by Fredrick Sanger to sequence the genome of bacteriophage φX174‚ 5375 nucleotides long (Sanger et al.‚ 1977). With the advances in sequencing technology‚ such as the application of gel electrophoresis and fluorescent
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The polymerase chain reaction (PCR) is a biochemical technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude‚ generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis‚[1][2] PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications.[3][4] These include DNA cloning for sequencing‚ DNA-based phylogeny‚ or
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