1 Class XII Chemistry Ch 5: Surface Chemistry Chapter Notes Top Concepts / Key learnings: 1. 2. 3. 4. 5. 6. Adsorption: The accumulation of molecular species at the surface rather than in the bulk of a solid or liquid is termed as adsorption. Adsorbate: The substance which is being adsorbed on the surface of another substance is called adsorbate. Adsorbent: The substance present in bulk‚ on the surface of which adsorption is taking place is called adsorbent. Desorption: The process of removing an
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EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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However relative quantification of gene expression using qRT-PCR is highly influenced by the expression stability of internal control or reference genes used for transcript normalization of target genes. The use of inappropriate or unstable reference gene(s) can seriously impact the transcript quantification results leading to false inferences or misinterpretations21‚22. Accurate normalization is thus necessary for obtaining biologically meaningful expression data and hence qRT-PCR analysis greatly
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Multifactorial or complex inheritance health conditions have a genetic component in origin and tend to run in the family depending on how close one is with the affected family member. They are caused by mutation in single genes and multiple contributing factors that cause these conditions. Such contributing factors include lifestyle‚ nutrition‚ alcohol use and smoking‚ pollution and certain medicines. The health conditions include heart diseases‚ diabetes‚ Alzheimer disease‚ birth defects such as
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Topic: Concepts of DNA Fingerprint and Forensic Analysis Table of Contents Introduction………………………………………………………………………………. 3 What is DNA Fingerprint...................................................................................................3 Forensic Science…………………………………………………………………………..4 DNA Fingerprint Methods Restriction Fragment Length Polymorphism (RFLP)…………………………………...4 Polymerised Chain Reaction (PCR)……………………………………………………...5 Short Tandem Repeats
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The Effects of Using the Heat Shock Treatment to Deliver a Vector in Genetic Transformation Introduction: In this experiment we are testing what is required for E. coli to successfully grow on LB (Luria Broth) plates with ampicillin and determining if any genetic transformation has occurred. By combining +pGLO LB and ampicillin we should get an ampicillin resistant gene and by using –pGLO we should create a non-genetic resistant bacteria. The pGLO plasmid has the GFP (green fluorescent protein)
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noun the deliberate modification of the characteristics of an organism by manipulating its genetic material. Genetic modification caused by human activity has been occurring since humans first domesticated organisms in 12 000 BC. Genetic engineering as the direct transfer of DNA from one organism to another was first accomplished by Herbert Boyer and Stanley Cohen in 1973. Advances have allowed scientists to manipulate and add genes to a variety of different organism and to induce a range of
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Cell Biology Study Guide- Exam 1_______________________________________________ EXAMPLES OF MULTIPLE CHOICE/ TRUE AND FALSE QUESTIONS 1. Which of the following about the Cell Theory is FALSE? a) All cells come from preexisting cells through division b) All organisms consist of one or more cells c) The cell is the basic unit of structure for all organisms d) All cells must contain organelles e) None of the above 2. A student in a cellular biology laboratory is faced with the task of detecting
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II.Results and Discussion A.Isolation of Agrobacterium tumefaciens: Six bacterial colonies were observed and screened on the selective medium. The bacterial colonies was able to characterized after 72 hours of incubation‚ bacterial colonies were visible with naked eyes on YEMA plates. The colonies appeared were medium sized bubble shaped‚ round‚ regular‚ sticky and white coloured colonies were observed. From these initial results‚ the isolated bacteria were tentatively identified as Agrobacterium
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However‚ those studies used PCR‚ pulsed-field gel electrophoresis (PFGE)‚ and culture methods to track organisms and antimicrobial resistance genes. WGS was often performed only on a small number of isolates and plasmids in these studies limiting the genomic resolution of the analysis. Additionally‚ there
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