#3. Name the types of microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside
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PATHOPHYSIOLOGY OF CHRONIC OSTEOMYELITIS Red text - signs & symptoms manifested by the client. * - as evidenced by the biopsy result Analysis: The client was diagnosed with chronic osteomyelitis on her 2nd confinement after she sought consult for a tender mass growth on her Right radius. Chronic osteomyelitis is an infection of the bone usually caused by extension of soft
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important role in survival of bacteria by allowing them to move into an environment that is most favourable for growth. In this exercise‚ rate of migration was determined using Nutrient soft agar with the test organisms Pseudomonas aeruginosa‚ Erwinia carotovora and Staphylococcus aureus while demonstration of motility and chemotaxis was done by microscopic assay and capillary assay using chemoeffectors like malate‚ glucose and streptomycin with E. carotovora as test organism. Results from the migration
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spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral and cervical smears‚ and‚ v. identify: Neisseria meningitides‚ Neisseria gonorrhoeae‚ Neisseria lactamica‚ and Moraxella catarhalis. Method: Lab manual page(s) 27-8 Results: Part A) A‚ B‚ C‚ D unknown
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identified using a method called Gram staining‚ whereby a stain is used to differentiate different types of organisms. Bacteria with and without a cell wall are classified as Gram-positive or Gram-negative‚ respectively. Bacterial types vary by shape and size. Coccus Coccus refers to spherical shaped bacteria that may live as individual cells‚ or pair up to form chains or bunches. Staphylococcus and Streptococcus are the most common bacteria in this group and usually are Gram-positive. These bacteria perform
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Journal of Thermal Analysis and Calorimetry‚ Vol. 94 (2008) 1‚ 75–81 FUNGICIDAL AND BACTERICIDAL ACTIVITY OF METAL DIETHYLDITHIOCARBAMATE FUNGICIDES Synthesis and characterization H. S. Rathore1‚ K. Ishratullah2‚ C. Varshney1‚ G. Varshney1 and S. C. Mojumdar3‚4* 1 2 Department of Applied Chemistry‚ Z. H. College of Engineering and Technology‚ Aligarh Muslim University‚ Aligarh 202002 India Indian Institute of Chemical Technology‚ Uppal Road‚ Hyderabad 500007‚ India 3 Department of Chemical
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giant cluster tightly packed together. 4. What is the difference between direct and indirect staining? (1) Direct staining stained bacteria with basic dye (methylene blue‚ carbol fuchsin‚ crystal violet‚ etc) which its coloring power is in positive ion‚ and reacts with the negatively charged cell. Thus‚ the bacterial cell will be stained with the basic dye’s color. In other words‚ direct staining
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onto a nutrient agar plate using the streak method. The plates were incubated for two days and the bacterium was able to grow. I studied the bacteria based of its physical characteristics of how it grew on the agar. I began my quest by conducting a Gram stain on my bacteria. I prepared a smear by placing a drop of water onto the center of a slide and then removed a loop full of bacteria to place within the water droplet. I use the aseptic treatment while doing this making sure to flame the inoculating
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subject to colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive‚ aerobic‚ coccus shaped bacteria. The fungal species were also identified‚ of which Aspergillus and Penicillium were dominant‚ followed by mucur‚ as sub dominant .This project aims to find out the water and soil quality of River
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for gram (+) or gram (-) microbes‚ while others aren’t specific. On the nonspecific TSA plate a large hazy white colonies was formed. On the other hand‚ there were also small orange colonies fewer in growth. Isolation onto a BAP would allow for a pure culture of just gram (+) which would be used to carry out the rest of the differential test. Isolation onto TSA/ MAC allowed for the growth of gram (-) and would be used for further test. Procedure: The catalase test was performed only on gram (+)
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