The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the ONPG
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Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water
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Homicide Investigation More work goes into a homicide investigation than what the media alludes to. Television shows like Law and Order‚ and CSI show an over glorified series of basic investigations as well as highly dramatic interrogation in which each homicide case is solved within a one-hour episode. In reality‚ a homicide investigation is 20 times more complex than what is aired on prime time television. Cases can take years to solve‚ and a large number remain open indefinitely. From start
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Methods of Social Investigation Emma Woodmansee ’Describe how you would plan and undertake an investigation into why some of this College’s students do not complete their degree courses.’ (You have been given only 100 to finance the study; and one term’s sabbatical.) Define the variables in the given title After a Research Statement has been formulated it is very important that the researcher defines any variables within it. A variable is any word whose meaning may be ambiguous or which could
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effective uses of: C a. informants. b. grid maps. c. crime analysis. d. surveillance. 7. Field notes represent the information which forms the content of the: D a. statements. b. follow-up investigation.
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– The importance of site investigation Page 2 – 4 1.02 – Trial Pits Page 4 1.03 – Auger holes Page 4 – 5 1.04 – Boreholes Page 5 – 7 1.05 – Soil sampling Page 7 1.06 – In-situ testing Page 8 1.07 – Quasi-static cone penetration testing Page 8 – 9 1.08 – Dynamic probing Page 10 1.09 – The Standard Penetration Test Page 11 1.10 – Field vane test Page 12 1.11 – Classifications of Soils Page 13 1.12 – Size range of grains Page 13 – 14 2.00 – Walk over survey
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Course Title: Criminal Investigation Unit Number: Unit 3 2. What are the similarities and differences between an arson investigation and a bombing investigation? Arson is the intentionally setting of fires to property‚ persons‚ and vehicles. Bombing can be defined as the offense of using a projectile‚ or explosive weapon to cause alarm‚ panic‚ danger‚ or death. There are many similarities and differences between the investigation of a bombing and the investigation of arson. The similarities
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An investigation into the specific heat capacity of a solid Introduction: In this experiment I investigated the specific heat capacity of brass and aluminium‚ in order to do this I arranged a series circuit with a heater‚ the heater would go inside the brass and aluminium and heat it for an amount of time in which I decided‚ I also used the voltmeter in parallel. This arrangement made me able to arrange the equation VIT= mcΔθ‚ since the electrical energy in would be equal to the mass times the
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Investigation Process There were several steps taken by the police in this homicide case. The main aspects of this homicide investigation process were the initial response to the crime scene‚ collection‚ documentation and preservation. Investigation Process Failure Main Factors There are three major mistakes police made in the investigation process. First of all‚ the crime scene investigation. After identifying the victims and finding 2 children remaining at the scene‚ the police officers
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