pigment containing chloroplasts. Upon adding a solvent mixture of hexane and acetone‚ the chlorophyll and carotene pigments can be extracted from the chloroplasts. Further decanting of the dark green fluid from the cloudy light green material will remove impurities from the extracted pigment. To perform thin layer chromatography‚ a dot of the extracted pigments is placed on a (TLC) plate. By using different ratios of Hexane: Acetone as a mobile phase in (TLC) we should be able to determine which solvent
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acquaint oneself with the fundamentals of the Aldol Condensation reaction by demonstrating the synthesis of dibenzalacetone (trans‚ trans-1‚5-Diphenyl-1‚4-pentadien-3-one) through the aldol condensation of acetone with benzaldehyde. The synthesis began by using a strong base to generate the acetone enolate ion. The ketone/enol tautomerization is an equilibrium process that produces little of the enol (ppm or less). However‚ any enol that formed quickly reacted with the strong base to form the enolate
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evaporate quickly and which will take longer to evaporate. Give reasons for your predictions. I predict that acetone‚ or C3H6O‚ will evaporate the quickest because there is no hydrogen bonds between oxygen and hydrogen. Therefore and London Dispersion bond is created which is very weak and doesn’t take much energy to break. The second liquid to evaporate will be isopropyl. C3H8O also like acetone doesn’t allow its oxygen to bond with any hydrogen therefore not creating hydrogen bonds. Also due to the
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example of self-condensation. In this lab‚ we were performing a mixed condensation‚ so this side reaction would not be favorable. The second reaction would occur if there was excess acetone in the reaction‚ causing acetone to further react with the desired final product. The last side reaction is due to not enough acetone in the reaction‚ only able to add to one side of the carbonyl and ultimately in the wrong position. This is why it was important to pay special attention to the amounts of reactants
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Experiment 1: Quantitative assessment of Some Cellular Constituents Summary: Euglena gracilis are unicellular organisms in the Protist Kingdom. They are known to have both plant and animal characteristics. Although‚ Euglena cells contain a variety of cellular constituents‚ their cellular constituents should be presented in equal ratio. The objective of this experiment is to determine Euglena’s cellular components in cells and then to establish their cellular constituents by comparing the experimental
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acetyl acetone allow shake then addition of 0.29 g of KMnO4 in 12.5cm3 of distilled water. A strong black of 2.19 g products was obtained then brown acac after recrystallization was obtained. The equation below simply explains the reaction: 5Mn2++MnO4-+15(CH3CoCH2COCH3)3→2H2O+7H++5Mn(CH3COCHCOCH3)3 Moles for Mn(acac)3: 0.00904mol MW of MnCl2.4H2O: 143.856 gmol Mass ofMnCl2.4H2O: 1.3 g Moles of acetyl acetone: 0.0499 mol Manganese (II) chloride tetra hydrate and acetyl acetone reacts
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2: Also‚ since the recycled acetone will not be pure‚ the pretreatment using recycled acetone should be tested to see the feasibility of the proposal. Response: After evaporation‚ only acetone together with some water is recovered; the solids‚ catalyst (sulfuric acid)‚ and other stuff cannot be evaporated. Then 50 % v/v acetone-water solution can be made by adding the necessary water. In principle‚ as there are no more volatile components in the system‚ except acetone and water‚ we did not have
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between the simple and fractional distillation‚ the fractional distillations for both n-propanol and acetone provided a better distillation. The data and graphs support the statement since the fractional graphs provided a steeper slope than the simple distillation. The steeper slope represent how fast the mixture are separated throughout the distillations. For example‚ the simple distillation for acetone the slope initially started at about 20 mL at about 65.5℃ and ended at about 30 mL at 101 ℃ in which
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Experiment : 1 Tittle : Preparation of bis(acetylacetonato)copper(II) complex Objective : To synthesis the bis(acetylacetonato)copper(II) complex Introduction : A complex ion is usually form with high charge density metal ion as a central and formation of coordinate covalent bond (dative bond) with high electron molecules or ions. These molecules or ions are functioning as ligands (electrophile in organic compounds). They are easily attracted by electrophile (electron deficiency
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PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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