for 3 minutes and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used
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CHEM 2123 and 2125 – Organic Chemistry Lab I and II GUIDELINES FOR WRITING LAB REPORTS INTRODUCTION Writing reports in organic chemistry lab may differ from the way it’s done in general chemistry. One goal of this course is to introduce you to the record keeping methods used in research labs. Such methods are designed to organize experimental data in a format similar to that required for publication in major scientific journals. Here are some important considerations that apply in research settings
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AP Chemistry Lab 2: Analysis of Alum Lab Objective: In this lab we will analyze alum by two techniques in order to verify its identity. The melting point and the mole ratio of hydrated water to anhydrous aluminum potassium sulfate will be determined. Lab Procedure: 1. Use a mortar and pestle to crush alum. 2. Pack the alum in capillary tube‚ and then fasten it to the thermometer. 3. Fasten the thermometer to the ring stand. 4. Immerse the bottom of the
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2KOH + H2 Table 8.6- Chemical reaction of copper strip and 4 mL silver nitrate Observations Precipitate formed on copper strip; precipitate was greenish brown in color. Reactants: Cu and AgNO3 Products: Cu(NO3)2 and Ag Balanced chemical equation 2AgNO3 + Cu Cu(NO3)2
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successful treatment of cancer is the development of resistance mechanisms to drug treatment. Various cellular changes that have been implicated in the development of drug resistance in cancer cells include: the increased expressions of P-glycoprotein (a multidrug transport protein) and multidrug resistance-associated protein (MRP-1); increased levels of the cellular detoxification protein‚ glutathione; and changes in the expression of apoptosis associated proteins such as Bcl-2‚ FasL and p53‚ which
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Electrochemical Cells Lab Report AP Chemistry Block 1 Analysis: The purpose of Part 1 of this laboratory is to construct a table listing the reduction potentials of a series of metal ions in order of ease of reduction. The series of half-cells is constructed by placing a piece of metal into a 1.0 M solution of its ions for each metal in the series. The metals are Cu‚ Fe‚ Pb‚ Mg‚ Ag‚ and Zn. The half-cells are connected by a salt bridge constructed of a strip of filter paper soaked in a solution
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a) Using a human cell of your choice‚ describe how organelles work together to make and secrete a protein (LO1. AC 1.1) An epithelial cell in the thyroid gland‚ called a thyrocyte forms spherical follicles that produce a protein called thyroglobulin. This is a globular protein that has a functional role in metabolism. The protein is used by the thyroid gland to produce thyroid hormones; thyroxine (T4) is an example of one of the hormones created. Thyroxine is formed by iodine binding to tyrosine
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N10/4/CHEMI/SP2/ENG/TZ0/XX 88106105 CHEMISTRY STANDARD LEVEL PAPER 2 Thursday 11 November 2010 (afternoon) Candidate session number 0 1 hour 15 minutes 0 INSTRUCTIONS TO CANDIDATES • • • • Write your session number in the boxes above. Do not open this examination paper until instructed to do so. Section A: answer all of Section A in the spaces provided. Section B: answer one question from Section B. Write your answers on answer sheets. Write your session number
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AP Chemistry Kinetics Lab Abstract: The purpose of this lab is to learn how to write law expressions‚ determine orders by graphs‚ and calculate rate constants. By determining the number of drops of sodium hypochlorite necessary to make a diluted food dye change to colorless in less than three minutes helps calculate the absorbance which can then be analyzed to find the pseudo rate constant and eventually leading to the rate constant and the rate law. The results show that the m and n are both 1st
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Lab 3 Exercise 3.1: Installing the DHCP Server Role‚ Questions 1-3. Question 1: What is the name of the computer you are working from? The Name of the computer is W2K8xx Question 2: What is the Default lease duration of a wired subnet type? Wired less duration will be 8 days Question 3: What are 3 recommendations listed in Server Manager for managing the DHCP Server role? 1. Increase fault tolerance by splitting DHCP scopes 2. Eliminate manual updates of DNS records by configuring dynamic
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