University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
Premium Protein Chromatography Analytical chemistry
Bio Lab Report Erica Patterson September 10‚2013 Intro to cellular and molecular Biology Lab Abstract: In the Biology Laboratory Manual by Darrell S. Vodopich and Randy Moore are results to a similar experiment. The studied the hypothesis of carbon dioxide production by yeast fed sugar is not significantly different than the carbon dioxide production by the yeast fed in protein. Their hypothesis is the one that has helped formulate ours. We also will be answering the same to questions “What
Premium Carbon dioxide Metabolism
We labeled the test tubes with numbers one through five. The first test tube was our calibration tube‚ here we added one milliliter (mL) of buffer‚ four mL of distilled water and three drops of chloroplasts using pipets. After‚ we created the solution for the second‚ third‚ fourth and fifth test tubes by adding one mL of buffer‚ three mL of distilled water one mL of DPIP an 3 drops of chloroplasts. However‚ we decided that test tube number two was going to be the test tube that was kept completely
Premium Photosynthesis Plant Oxygen
the fire of origin (tests needs to be run to confirm). The crazed glass (by lab results) indicated that crazing is the result of rapid cooling of hot glass by the application of water. The protected surface shows there was an object on the floor protecting the area‚ and there is the shape of a body that was there during the fire. The puddle-shaped burn often occurs in areas of intense burning‚ with or without the presence of ignitable liquids in the burn wood flooring (tests need to be run to confirm
Premium Chromatography Gas chromatography Light
Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
Premium Bacteria Escherichia coli Gram staining
Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
Premium Plasmid Escherichia coli Agar plate
on hydrostatics‚ or is also known as fluid statics (fluid at rest) within the fluid mechanics field of study. This condition explains that in a stable condition‚ the fluid is at rest. The use of fluid in doing work is known as hydraulics‚ and the science of fluid in motion is known as fluid dynamics. INTRODUCTION The natural nature of fluids are they cannot remain stationary under the application of shear stress. However‚ fluid can apply force normal to any surface contacting it. If the fluid
Premium Fluid mechanics Force Fluid dynamics
Biology‚ Bio 110; October 1‚ 2014 Observing Membrane Structure and Observing Effects of Chemical stress on Membrane Crystal Eve Lopez‚ Dr. Barua Madhabi Keywords: beet root model system‚ spectrophotometer‚ betacyanin‚ cellular membrane‚ phospholipid Abstract The cellular membrane separates and protects the cell acting almost as a wall. Depending on what stressors there are the cellular membrane can become damaged. The objective of this experiment was to examine the struc
Premium Cell membrane Protein Cell
Enzyme Lab Experiments Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme? Materials: G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar Solo Cups Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide Hypothesis: 1. If we change the environment via temperature the glow stick will Its intensity will change 2. If hydrogen peroxide is added to a certain food liver
Premium Oxygen Enzyme Hydrogen peroxide
3.10 Results of Experiments After performing all the experiments with predetermined values of process parameters‚ the tensile strength is measured and the micro hardness is measured. The results are given in the table 3.5. Table: 3.5 Ultimate Tensile Strength and Micro hardness of SMAW & TIG for Experimentation Exp. No Groove Angle (Degree) Root Face (mm) Root Gap (mm) SMAW TIG UTS (MPa) Vickers Hardness (Hv0.1) UTS (MPa) Vickers Hardness (Hv0.1) 1 30 1 0 304 209 358 220 2 30 1.5 1 275 210
Premium Chemistry Chemical reaction Temperature