consists of a large domain of prokaryotic microorganism in which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly with my results
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When well is loaded with antibiotics‚ it diffuses in the medium and inhibits the growth of organism. There is logarithmic reduction in antibiotic concentration. The zone of inhibition of bacterial growth around each well is measured and the susceptibility is determined. Medium-Muller Hinton Agar (3.8gm/100ml of distilled water) was prepared
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microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. Broth culture #72 was randomly selected and subjected to qualitative tests for identification. It is suggested that culture #72
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Introduction Pneumonia is an inflammation of the lungs which can develop from bacteria‚ a virus‚ or toxins and is the sixth leading cause of death in the states. People who are at a higher risk in contracting the disease are the elderly‚ those who are hospitalized for other conditions‚ those with coughs after a stroke‚ smokers‚ those who suffer with malnutrition‚ alcoholics‚ those who have bronchitis‚ those with sickle-cell anemia‚ those undergoing radiation treatments or chemotherapy‚ and those
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the gel electrophoresis with agarose followed by isolation of fragments from the gel‚ bacterial plasmid isolation‚ restriction mapping and during class excursions I got to see the use of this techniques on a large scale like the production of antibiotics using the process of fermentation
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D.M. and S.W. Joseph‚ Accessed 5 April 2011‚ Pathogenic Microbiology‚ http://ww w.life.umd.edu/classroom/bsci424/PathogenDescriptions/Enterococcus.htm‚ August 2000. 4. Winfrey‚ M.R.‚ M.A. Rott‚ S.J Anglehart‚ W.R. Schwan‚ B.C. Taylor‚ D.J. Grimes‚ and R.M Burns. 2010. Laboratory manual for fundamentals of microbiology: isolation and identification of unknown organisms from mixed culture‚ p. 21-1 – 21-5 and 1 – 4 . Department of Microbiology‚ UW-La Crosse‚ WI.
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infecting an animal with Pseudomonas aeruginosa protected the animal against Bacillus anthracis. Later‚ the word “antibiosis” (against life) for this inhibition and called the inhibiting substance “Antibiotic”. As researchers found out more and more about these chemicals they were able to discover that antibiotics are chemicals or natural substances such as bacteria‚ fungi‚ as well as synthetic drugs; that inhibit or kill microbes in small amounts. Chemotherapeutic agents are antimicrobial chemicals (natural
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to inflammation‚ systemic stress‚ rhabdomyolysis‚ life support treatment and/or urinary catheter insertion. Methods Prospective‚ single-centre‚ observational study conducted in patients with severe trauma and without a history of UTIs or recent antibiotic treatment. The 24-hour
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History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media
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geographically separate areas of Jordan‚ Micrococcus luteus and Staphylococcus aureus were rapidly killed. Over the 3-week incubation period‚ the number of specific types of antibiotic-producing bacteria increased‚ and high antimicrobial activity (MIC‚ ∼10 μg/ml) was observed in methanol extracts of the inoculated red soils. Antibiotic-producing microorganisms whose numbers increased during incubation included actinomycetes‚ Lysobacter spp.‚ and Bacillus spp. The actinomycetes produced actinomycin C2 and
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