Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology‚ 1822–1895) There
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Determining an Unknown Through Deferential Stains and Biochemical Tests Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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Bacillus thuringiensis Bacillus thuringiensis Spores and bipyramidal crystals of Bacillus thuringiensis morrisoni strain T08025 Scientific classification Kingdom: Eubacteria Phylum: Firmicutes Class: Bacilli Order: Bacillales Family: Bacillaceae Genus: Bacillus Species: thuringiensis Binomial name Bacillus thuringiensis Berliner 1915 Contents Introduction Use in pest control Genetic engineering for pest control Usage Advantages Safety Limitations to Bt crops
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Bacillus Cereus Bacillus cereus is a rod-shaped gram- positive bacillus that can be found in food‚ dust‚ dirt and sometimes soil. It is an aerobe and a facultative anaerobe that can form spores‚ which if activated at any point can begin to germinate and produce toxins. These spores that form from B. cereus are be resistant to heat and chemicals. B. cereus is found in approximately 25% of food sampled (3). Foods that are commonly associated with B. cereus are potatoes‚ rice‚ meat‚ vegetables
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Microorganisms are classified according to their structure. By means of flow charts‚ diagrams and tables explain the differences between Viruses‚ Bacteria‚ Cyanobacteria‚ Achaea and Fungi. Bacteria or bacterium are unicellular microorganisms. They are essentially only a few micrometres long and form of various shapes including the spheres‚ rods and spirals. A BACTERIAL CELL Illustration courtesy of Wikipedia. A Virus (from the Latin noun virus‚ meaning toxic or poison) is a sub-microscopic
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carboxylase for CO2 fixation Gas vacuoles: protein covered cylinder Magnetosomes: Iron oxide kills H2O2 Nuclear Area: no nucleus has nucleoid Ribosomes make protein Endospores: resting‚ not growing‚ resistant to desiccation‚ heat‚ chemicals. Bacillus‚ Clostridium a. Sporulation: Endospore formation b. Germination: return to vegetative state c. After endospore forms bacterial cells die d. Dormancy: Sporulation occurs surrounding cell dies away. Temp. gets better it germinates and continues
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themselves to substrate molecules and lowering the activation energy of a reaction (Alberts et.al‚ 2010 p.90). The purpose of the lab was to test how temperature will affect the rate at which starch hydrolyzed. In the experiment conducted‚ bacterium; Bacillus Licheniformis and the fungus; Aspergillus Oryzae was used to examine how changes in temperature of the enzymes affects the rate of the reaction with starch. Amylase is an enzyme‚ found chiefly in saliva and pancreatic fluid‚ that converts starch
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you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques: 1. Streak plate method for colony isolation 2. Aseptic transfer techniques First the streak plate methods. In Microbiology it is often necessary to isolate pure colonies
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