bacteria that retain the color from the primary stain is called gram-positive. Bacteria stain differently because of chemical and physical differences in the cell wall. Procedure For this lab we used three cultures; Escherichia Coli‚ Bacillus Subtilis‚ and Staphylococcus Epidermidis. Using a different slide for each sample we started by preparing and fixing each slide. Next we covered the slide with crystal violet for 30 seconds then rinsed with water. Next we covered the slide with Grams
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found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as the hay bacillus or the grass bacillus. It is gram and catalase positive. It is rod-shaped‚ with the ability to form a tough‚ protective‚ shield around itself. It can tolerate extreme environmental conditions‚ and is an obligate aerob. The antibiotic that B. subtilis is resistant to is Tetracycline. When a bacteria is resistant to an antibiotic‚ it means
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Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials
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work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly with my results but Lysol was only 4mm more sustainable then Windex. Lysol with the biggest zone of inhibition of 28mm allowing this disinfectant to kill the most Bacillus subtilis bacteria. Still‚ Lysol was succeeded to work‚ but
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ISOLATION AND SCREENING EXTRACTED FROM GOTU KOLA (Centella asiatica) ROOTS EXTRACT Submitted by: ALIANA CLARENCE B. JAMERO IV – REG A Research Paper Presented to the Faculty of Gusa Regional Science High School Region X In Partial fulfilment of the requirements For Research 2 Submitted to: ERMA S. DAPIN Adviser March 2014 APPROVAL SHEET The research paper hereto attached‚ entitled; “ISOLATION AND SCREENING EXTRACTED FROM GOTU KOLA (Centella asiatica) ROOTS EXTRACT”
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Antibiotics Vs Tea Tree Oil To determine the effect of various different antibiotics and Tea Tree oil on the Bacillus subtillis different types of bacteria. I will test six antibiotics and the Tea Tree oil on Bacillus subtilis. I will test tetracycline‚ chloramphenicol‚ ampicillin‚ sulphatriad‚ penicillin G and Streptomycin. I would also like to see if Bacillus subtillis shows resistance to Antibiotics or to Tea Tree oil. The use of penicillin is being reduced because of the resistance
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decided to see if the stories about garlic are true. You can get a look at her project display in the figure. Figure: Project display for “Garlic and bacteria inhibition.” Hypothesis I believe that garlic will inhibit the growth of the bacillus subtilis bacteria‚ but not as effectively as antibiotics and disinfectants. Independent variables Bacteria-inhibiting agents Dependent variables Amount of bacteria incubated Controls • Bacteria used • Technique and time of incubation Experimental
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more than 100 phytochemicals. It is used as sugar substitute. The extracts from leaves are used for the microbial assay and study the antimicrobial activity. The microbes used for the testing the activities are E.coli‚ Staphylococcus aureus‚ Bacillus subtilis‚ Salmonella typhi‚ Aspergillusniger‚ Candida parapsilosis‚ Trichophyton rubrum. With different concentrations of the extracts starting from 2.5µg/ml‚ 5.0µg/ml‚ 7.5µg/ml‚ 1.0µg/ml and the highest activity is shown by Staphylococcus aureus and
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Amanda Lab Report 1 Microbiology Laboratory Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories
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experiment carbohydrate catabolism was first tested under aerobic conditions. We began this by drawing three sections on the bottom of the petri plate‚ dividing it into three sections. Using a sterile loop‚ we streaked a single line of each culture Bacillus subtilis‚ Pseudomonal aeruginosa‚ and Escherichia coli‚ to each of the separate sections. The plate was incubated until the next lab period‚ where we then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table 1)
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