of biological system is usually preferred as the pH system‚ which determines the pH level of dilute aqueous solutions. In this laboratory‚ the data collected from the experiment will be used to graph the titration curves which help to identify an unknown amino acid. IntroductionAmino acids are simple monomers which are strung together to form proteins. Amino acids play a key cellular role in structure and function. Proteins themselves participate in nearly every physiological event in the cell. Since
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Standardization and Titration of an Unknown Organic Acid Overview: Methods for counting the number of molecules in a sample is a major emphasis of laboratory work. In this experiment we will use the method of titration to count the number of acid molecules in a solution. Measuring mass is a relatively easy procedure to do in the lab (although a balance is expensive). Counting the number of particles requires more effort. Molecular counting can be done by setting an unknown amount of a substance equal to
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Classification for Functional Groups Alcohols Lucas test (Differentiates primary‚ secondary‚ and tertiary alcohols) Reagent: ZnCl2 in conc. HCl Observation: Rate of reaction (tertiary alcohol> secondary alcohol> primary alcohol) Procedure: 2mL Lucas Reagent in test tube+ 3-4 drops of alcohol‚ stopper‚ shake vigorously‚ NOTE time required (less than 10m mins only) to form an emulsion or separate layers. Oxidation (Confirms if alcohol is oxidizable: presence of H in C-OH bond) Reagent:
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Using a Dumas method‚ you are asked to identify the unknown pure liquid by comparing the molar masses of the given possible substances (ethanol‚ methanol‚ isopropanol‚ acetone). Introduction Dumas Method allows us to measure the molar mass of the substance‚ using the Ideal Gas Law. The Ideal Gas Law‚ PV=nRT‚ explains the behaviour of the gases that are near 100kPa and in the room temperature. This equation shows the product of the pressure(P) and volume(V) equals to the product of the number of moles(n)
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References: - 1. Leboffe‚ Michael J. and Pierce‚ Burton E.(2012). Breif Microbiology Laboratory Theory and application Second Edition‚ Common Aseptic Transfers and Inoculation Methods (pp. 26-38). Englewood‚ CO: Morton Publishing. 2. Leboffe‚ Michael J. and Pierce‚ Burton E.(2012). Breif Microbiology Laboratory Theory and application Second Edition‚ Streak Plate Methods of Isolation (pp. 39-44). Englewood‚ CO: Morton Publishing.
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to cocoa bottom and an unknown girl notes to help on coursework. An unknown girl A traditional indian market scene‚ set in the evening‚ it was to hot to have markets in the day‚ lit up with neon lights She tries to capture the moment‚ she is getting involved in the culture by having her hand hennad. It is a darker shade of your own skin‚ suttle. She is watching this girl ‘icing my hand’(verb(icing)) ‘in the evening bazaar for a few rupees’ india money Unknown girl refers to the lady
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Tuan Tai 1 Duong Tran Tuan Tai Professor Pham Quoc Loc ENGL - 1302 17 September 2014 Some nice quotes from unknown authors In my view‚ everyone love reading always notes their favorite quotes for their life’s guideline. To me‚ I am not really love reading but I love collecting the nice quotes. The quotes bring many meaningful‚ some are evident but some are vague. In addition‚ I also like to collect quotes from the Internet because I do not need to read whole a book and some of them never appear
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determined that Unknown A‚ B‚ C‚ and D are to be identified as the Sprite‚ burger‚ fries‚ and bun‚ respectively. Each Unknown provided evidence that we eventually used to match to each element of the meal. Along with the lab data‚ our prior knowledge also led us to our results. We were able to determine that Unknown A is the Sprite due to the fact that every test was negative except for simple sugars‚ a macromolecule that the majority of sodas contain. We determined that Unknown C were the
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Thin Layer Chromatography of Unknown Samples and Paprika By: Uyen Huynh Date: June 18‚ 2014 Lab Partners: Philip Murray Professor and section: M. Pandey‚ CHM2210L.601 Data: Analysis of Drug Store Items Knowns Rf Values Aspirin .91 cm Acetaminophen .85 cm Ascorbic Acid .86 cm Unknown .97 cm Identity of unknown mixture Acetaminophen Analysis of Paprika Pigments Spot Number Rf Color 1 1 Red-orange 2 1 Red-orange Calculations
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Determination of An Unknown Amino Acid From Titration Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96‚ and the pKa’s found experimentally were 2.0‚ 4.0‚ and 9.85. The accepted pKa values were found to be 2.10‚ 4.07‚ and 9.47. The molecular weight was calculated to be 176.3 while the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be glutamic acid‚
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