INTRODUCTION This scientific report is to discuss the results of an experiment being conducted on the effect that antibiotics have on the growth of bacteria. This will be done through the use of microbiology. Microbiology is a science in the department of biology‚ particularly microorganisms. These include; bacterium‚ fungi‚ protozoa‚ algae and viruses. This is an important subject to study as microorganisms can be found everywhere‚ covering all matters of different surfaces even including the human
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Materials 1. Nutrient agar plates of a) Sarcina lutea and Serratia marcescens b) Bacillus subtilis and Escherichia coli c) Streptococcus fecalis and Pseudomonas fluoroscens 2. 24 hour pour plate preparation of a) Serratia marcescens b) Sarcina lutea c) Bacillus subtilis d) Escherichia coli 3. 24 hour agar slant of the following cultures incubated at 25ºC a) Bacillus subtilis b) Escherichia coli c) Serratia marcescens d) Pseudomonas fluoroscens 4. 48
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of the growth patterns of Bacillus subtilis colonies on agar plates”. FEMS Microbiology Ecology 13: 159-167 4. Anna Csillag‚ 1972. “Appearance of acid-fast rods in cultures of mycococcus luteus”. Tubercle and Lung Disease 53: 221-225 5. James A. Bastock‚ Michelle Webb and Jane A. Grasby‚ 2007. “The pH dependence of the Escherichia coli RNase HII-catalysed Reaction Suggests that an Active Site Carboxylate Group Participates Directly in Catalysis”. Journal of microbiology 368: 421-433 6. Lloyd D. Witter
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UNIVERSITY OF DAR –ES-SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCES DEPARTMENT OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY MC206: FOOD MICROBIOLOGY PRACTICALS PRACTICAL 1 MICROORGANISMS IN THE ENVIRONMENT GROUP #:1 NAME: DUSENGEMUNGU Léonce REG #: 2011-04-07086 COURSE INSTRUCTOR: Dr Mugassa S.T Rubindamayugi
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed
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which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial species from various environmental sources is important in all branches of microbiology since bacteria are ubiquitous and live in microbial communities of mixed populations. Populations in microbial communities or ecosystems may interact and cooperate in their efforts to obtain nutrients from the environment with the waste products
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References: 1. Tortora G.J.‚ Funke B.R.‚ Case C.L.‚ 2010. Microbiology: An Introduction. 10th ed. CA: Pearson Education Inc. 2. B. John.‚ 2000. Effect of pH on microbial growth. [Online] Available on <http://www.brooklyn.cuny.edu/bc/ahp/CellBio/Growth/MGpH.html> [Accessed on 5th July‚ 2012] 3. Farlex.‚ 2012
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Egypt. Acad. J. Biolog. Sci.‚ 5(1): 1-9 (2013) Email:egyptianacademic@yahoo.com Received: 20/1/2013 G. Microbiology ISSN: 2090-0872 www.eajbs.eg.net Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia africana. Al-mahi Idris‚ Ietidal Al-tahir and Eihab Idris* Faculty of Science‚ University of Khartoum. P.O. Box 321‚ Postal Code 11115‚ Khartoum‚ Sudan. *Corresponding author: E-mail: eihabidriss@gmail.com ABSTRACT The aim of this study was to identify the endophytic
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com/botanical_biotechnology_biology_chemistry/biotechnology_methods/microbiology/bacterial_staining.php * Preparation of Bacterial Smear and Simple Staining Technique http://samples.jbpub.com/9780763795573/95573_CH04.pdf * Preparing a Smear and Simple Staining http://facstaff.gpc.edu/~ddonald/biolab/smearstain.htm * Bacterial Smears and Simple Stains http://www.highlands.edu/academics/divisions/scipe/biology/labs/rome/bacterial_smears_and_stains.htm * Microbiology Techniques http://www2.hendrix.edu/biology/CellWeb/Techniques/microstain
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