Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology‚ 1822–1895) There
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as are their abilities to develop and adjust to altering environments. That is why it is so significant to be competent to identify microorganisms. This study was conducted by applying techniques and experiments cultured in microbiology lab for the identification of the unknown microorganism. Introduction: Identification of bacteria is significant because they are not only beneficial but potentially hazardous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding
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purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain‚ used
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Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday‚ September 18‚ 2008OBJECTIVESIn this lab experiment two different types of bacteria‚ Escherichia coli and Staphylococcus aureus‚ were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. Resulting data was analyzed to provide understanding of the use of differing culture media and conditions for bacterial growth. RESULTSFour different agar types were used in this
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Morales‚ Marinel M. Dr. Annie Cu Gallardo BSE-Biological Sciences III-3 Microbiology (Lecture) Microbiology Written Report REPORT NO. 1 Prokaryotes: Bacteria Most of us have been conditioned to think of bacteria as invisible‚ potentially harmful little creatures. Actually‚ relatively few species of bacteria cause disease in humans‚ animals‚ plants‚ or any other organisms. In fact‚ all organisms made up of eukaryotic cells probably evolved from bacteria-like organisms‚ which were some
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Gram Characteristics of Bacteria Introduction Bacteria are interesting little critters in that they are not easily visualized without some sort of stain. In classical methodology‚ two different kinds of stains have been used to identify bacteria: acidic and basic dyes. Acidic dyes are so called because they consist of a salt that has a cation that transfers no color‚ but the anion portion is colored and does give off color. Basic dyes consist of an anion that does not give off any color and a cation
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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Determining pH Using a Red Cabbage Farnoosh Mazarei Food Microbiology April 22‚ 2011 Purpose: To test the pH level of many common household object by use of the juice from a red cabbage. Background: pH is the measure of the acidity or basicity of a solution. The pH scale ranges from 1 to 14 with 1 through 6 being acidic and 8 through 14 being basic; 7 is considered neutral. The use of the red cabbage‚ or red cabbage juice is because red cabbage can be used as an acid/base indicator
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disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous in his stool. The patient had no pain but mild discomfort during bowel movements. The doctor noted inflammation of the lining of the rectum. The
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