The Effects of varying p-Nitrophenyl Phosphate concentrations on Alkaline Phosphatase Activity Nathan Overholt Louis Settembrino March 5‚ 2002 Abstract: The enzyme Alkaline Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time assay was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm‚ and the Km was found to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction:
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AIR POLLUTION IN THE PHILIPPINES Guides and Tips Home / Blog / Air Pollution in the Philippines Air pollution is a common and frequent problem nowadays. When you are on your way to work‚ you pass by your neighbors’ backyard where he is currently burning their garbage from yesterday‚ then you come across a smoke-belching public utility vehicle‚ then a guy beside you lighted a cigarette and started suffocating you with second-hand smoke. You cannot always depend on your handkerchief on covering
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THE TUBBATAHA REEF The name ’Tubbataha’ is a Samal word for "long reef exposed at low tide". Samals are seafaring people of the Sulu Sea. Cagayanen people who are more geographically associated with Tubbataha Reefs referred the Park as ’gusong’. Location Tubbataha Reefs Natural Park (TRNP) lies in the middle of the Sulu Sea and falls under the political jurisdiction of Cagayancillo‚ an island municipality situated 130km to the north. The park is around 150km southeast of Puerto Princesa City -
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pH and Living Systems I. Purpose: To observe the effects of pH change on an organic molecule. II. Materials: pH paper Droppers Ammonia Beakers (50ml) Paper towels water Glass stirring rods lemon juice forceps III. Procedure: Part 1: Initial pH testing 1) First use the wide range pH paper to test the pH of the liquids given. 2) When you test with the wide range paper first (which reads pH from 0-13) be sure
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determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient showing that as the percentage transmission will decrease with an increasing pH. This happens
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After 4 gram stains‚ the slide was blot dried and observed under microscope at 100x with oil immersion. The slide either appear in pink (gram-negative) or purple (gram-positive). Catalase Test: Catalase test enzyme detection of microorganisms‚ catalase hydrolysis hydrogen peroxide (H2O2) into H2O and O2. The catalase test was performed by used a sterile transfer loop been flamed to remove small amount of bacteria from slant agar and placed on the slide. Then
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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Observing Enzyme Activity Purpose: The purpose of this experiment was to test whether the pH affected the enzyme reaction rate. Hypothesis: If the enzyme is in a basic solution‚ then it will react faster because the enzyme (catalase) reacts better in basic solutions. Materials: 10 potato cubes (1 cm3) -Pipet Baking soda solution -50 ml glass beaker Bleach Water Lemon juice Vinegar 5 glass test tubes Drying rack Timer Graduated cylinder Hydrogen peroxide Procedure:
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only as an annex named‚ Marikina High School Annex. By the year 1993‚ Mrs. Lagrimas B. Garcia‚ the school principal‚ made extra effort on achieving full independence of the Marikina High School Annex to give birth to the school‚ Parang High School (PHS). PHS had been through a lot of changes since the School Year 1996-1997. Hon. Congressman Romeo DC Candazo‚ Hon. Bayani BH Fernando and Dr. Perla B. Menguito made a big impact on the transformation of the school’s image and into a conductive learning environment
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Aim: To observe the effect of temperature on the rate of the iodine clock reaction using ammonium persulfate Assessment criteria: Design Variables: Table 1.1: List of dependent and independent variables. S.No | Dependent variables | Independent variables | Controlled variables | 1. | Rate of reaction | Temperature | Concentration | 2. | - | - | Pressure | 3. | - | - | Volume | Hypothesis: The iodine
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